Fabrication and tissue engineering application of a 3D PPF/DEF scaffold using Blu-ray based 3D printing system

Abstract

Micro-stereolithography (MSTL) among various 3D printing technologies reaches high resolution by using a focused laser beam, and therefore it can be used to fabricate objects that have feature sizes of tens to hundreds of micrometers. To fabricate a scaffold for the tissue engineering, we used a Blu-ray based MSTL system which is simpler and more compact than the conventional MSTL system. We selected a biodegradable photopolymer, Poly (propylene fumarate)/diethyl fumarate (PPF/DEF), as the construction material, and progressed post-curing to strengthen the fabricated scaffold. We seeded MC3T3-E1 pre-osteoblasts on the fabricated PPF/DEF 3D scaffolds and cultured them in a multi-stimulus bioreactor system which mimics the in-vivo shear flow environment and simultaneously supplies a magnetic field to improve cell proliferation. A cell culture result showed the superiority of combining our bioreactor system with the PPF/DEF 3D scaffold. The combination of 3D scaffold fabricated by Blu-ray based MSTL and a multi-stimulus bioreactor system may be a valuable tool for bone tissue regeneration.

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Authors

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Correspondence to Jin Woo Lee.

Additional information

Recommended by Associate Editor Won Hyoung Ryu

Jae-Hun Kim received his B.S. and M.S. degrees in Mechanical Engineering from the Korea Polytechnic University in 2012 and 2014. His research interests include 3D printing technology and its application to biomedical field such as medical device, tissue engineering and local drug delivery.

Jin Woo Lee received his Ph.D. degree in Mechanical Engineering from POSTECH in 2009. He is currently an Assistant Professor in the Department of Molecular Medicine at Gachon University. His research interests are realization of 3D micro-environment using 3D printing, and synthesis and modification of biomaterials.

Won-Soo Yun received his Ph.D. degree in Mechanical Engineering from POSTECH in 2000. He is currently a Professor in Department of Mechanical Engineering at Korea Polytechnic University. His research interests are on the advanced manufacturing system and tis biomedical applications.

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Kim, JH., Lee, J.W. & Yun, WS. Fabrication and tissue engineering application of a 3D PPF/DEF scaffold using Blu-ray based 3D printing system. J Mech Sci Technol 31, 2581–2587 (2017). https://doi.org/10.1007/s12206-017-0456-y

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Keywords

  • 3D printing
  • Blu-ray
  • Scaffold
  • Bioreactor
  • Multi-stimuli