Skip to main content
SpringerLink
Log in

Influence of Supramolecular Chiral Hydrogel on Cellular Behavior of Endothelial Cells Under High-Glucose-Induced Injury

  • Published:
Journal of Shanghai Jiaotong University (Science) Aims and scope Submit manuscript

Abstract

In order to manage the impaired wound healing of diabetic chronic wounds induced by high-glucose-damaged endothelial cells, a glucose-induced injured endothelial cell model is established in this research to explore the regulatory effects of a supramolecular chiral hydrogel on injured endothelial cells. Cellular behaviors of endothelial cells under different culture conditions are evaluated by CCK-8, 5-ethynyl-2′-deoxyuridine (EdU) staining, adhesion, and Transwell assays. The expression levels of angiogenesis-related markers, including nitric oxide, endothelial nitric oxide synthase (eNOS), and vascular endothelial growth factor (VEGF), are also assessed by enzyme-linked immune sorbent assay (ELISA) and the polymerase chain reaction (PCR). The results demonstrate that the left-handed chirality of the supramolecular chiral hydrogel can promote cell proliferation and enhance the adhesion and migration ability of impaired endothelial cells. Moreover, enhanced nitric oxide synthesis and elevated expression of eNOS and VEGF are observed in cells in the left-handed chiral environment. Thus, the left-handed supramolecular chiral hydrogel can regulate the cellular behavior of high-glucose-injured endothelial cells, including cell proliferation, adhesion, migration, and angiogenesis, offering the potential to promote diabetic wound healing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. EMANUELLI T, BURGEIRO A, CARVALHO E. Effects of insulin on the skin: Possible healing benefits for diabetic foot ulcers [J]. Archives of Dermatological Research, 2016, 308(10): 677–694.

    Article  Google Scholar 

  2. MALONE-POVOLNY M J, MALONEY S E, SCHOENFISCH M H. Nitric oxide therapy for diabetic wound healing [J]. Advanced Healthcare Materials, 2019, 8(12): 1801210.

    Article  Google Scholar 

  3. PATEL S, SRIVASTAVA S, SINGH M R, et al. Mechanistic insight into diabetic wounds: Pathogenesis, molecular targets and treatment strategies to pace wound healing [J]. Biomedicine & Pharmacotherapy, 2019, 112: 108615.

    Article  Google Scholar 

  4. FRANCESKO A, PETKOVA P, TZANOV T. Hydrogel dressings for advanced wound management [J]. Current Medicinal Chemistry, 2018, 25(41): 5782–5797.

    Article  Google Scholar 

  5. WICHTERLE O, LÍM D. Hydrophilic gels for biological use [J]. Nature, 1960, 185(4706): 117–118.

    Article  Google Scholar 

  6. FRANCESKO A, FERNANDES M M, ROCASALBAS G, et al. Polymers in wound repair [M]//Advanced polymers in medicine. Cham, Switzerland: Springer, 2015: 401–431.

    Google Scholar 

  7. DU X W, ZHOU J, SHI J F, et al. Supramolecular hydrogelators and hydrogels: From soft matter to molecular biomaterials [J]. Chemical Reviews, 2015, 115(24): 13165–13307.

    Article  Google Scholar 

  8. MOGOŞANU G D, GRUMEZESCU A M. Natural and synthetic polymers for wounds and burns dressing [J]. International Journal of Pharmaceutics, 2014, 463(2): 127–136.

    Article  Google Scholar 

  9. BENSON K, GALLA H J, KEHR N S. Cell adhesion behavior in 3D hydrogel scaffolds functionalized with D- or L-aminoacids [J]. Macromolecular Bioscience, 2014, 14(6): 793–798.

    Article  Google Scholar 

  10. SUN T L, HAN D, RHEMANN K, et al. Stereospecific interaction between immune cells and chiral surfaces [J]. Journal of the American Chemical Society, 2007, 129(6): 1496–1497.

    Article  Google Scholar 

  11. LIU G F, ZHANG D, FENG C L. Control of three-dimensional cell adhesion by the chirality of nanofibers in hydrogels [J]. Angewandte Chemie International Edition, 2014, 53(30): 7789–7793.

    Article  Google Scholar 

  12. GREEN D W, LEE J M, KIM E J, et al. Chiral biomaterials: From molecular design to regenerative medicine [J]. Advanced Materials Interfaces, 2016, 3(6): 1500411.

    Article  Google Scholar 

  13. DENG J, WU S, YAO M Y, et al. Surface-anchored poly(acryloyl-L(D)-valine) with enhanced chirality-selective effect on cellular uptake of gold nanoparticles [J]. Scientific Reports, 2016, 6: 31595.

    Article  Google Scholar 

  14. LI P, YIN Z Q, DOU X Q, et al. Convenient three-dimensional cell culture in supermolecular hydrogels [J]. ACS Applied Materials & Interfaces, 2014, 6(10): 7948–7952.

    Article  Google Scholar 

  15. LIU J Y, YUAN F, MA X Y, et al. The cooperative effect of both molecular and supramolecular chirality on cell adhesion [J]. Angewandte Chemie International Edition, 2018, 57(22): 6475–6479.

    Article  Google Scholar 

  16. WEI Y, JIANG S J, SI M T, et al. Chirality controls mesenchymal stem cell lineage diversification through mechanoresponses [J]. Advanced Materials, 2019, 31(16): 1900582.

    Article  Google Scholar 

  17. JANSEN F, YANG X Y, FRANKLIN B S, et al. High glucose condition increases NADPH oxidase activity in endothelial microparticles that promote vascular inflammation [J]. Cardiovascular Research, 2013, 98(1): 94–106.

    Article  Google Scholar 

  18. LV K, ZHANG L, LU W S, et al. Control of supramolecular chirality of nanofibers and its effect on protein adhesion [J]. ACS Applied Materials & Interfaces, 2014, 6(21): 18878–18884.

    Article  Google Scholar 

  19. SALDIVAR J C, HAMPERL S, BOCEK M J, et al. An intrinsic S/G2 checkpoint enforced by ATR [J]. Science, 2018, 361(6404): 806–810.

    Article  Google Scholar 

  20. WANG X Y, WANG X F, WANG M Z, et al. Probing adsorption behaviors of BSA onto chiral surfaces of nanoparticles [J]. Small, 2018, 14(16): 1703982.

    Article  Google Scholar 

  21. CHEN K, SHENG Y B, WANG J, et al. Chirality-dependent adsorption between amphipathic peptide and POPC membrane [J]. International Journal of Molecular Sciences, 2019, 20(19): 4760.

    Article  Google Scholar 

  22. HYNES R O. The extracellular matrix: Not just pretty fibrils [J]. Science, 2009, 326(5957): 1216–1219.

    Article  Google Scholar 

  23. PALUCH E K, ASPALTER I M, SIXT M. Focal adhesion-independent cell migration [J]. Annual Review of Cell and Developmental Biology, 2016, 32: 469–490.

    Article  Google Scholar 

  24. LIM J, CHOI A, KIM H W, et al. Constrained adherable area of nanotopographic surfaces promotes cell migration through the regulation of focal adhesion via focal adhesion kinase/Rac1 activation [J]. ACS Applied Materials & Interfaces, 2018, 10(17): 14331–14341.

    Article  Google Scholar 

  25. GERHARDT H. VEGF and endothelial guidance in angiogenic sprouting [J]. Organogenesis, 2008, 4(4): 241–246.

    Article  Google Scholar 

  26. VOKES S A, YATSKIEVYCH T A, HEIMARK R L, et al. Hedgehog signaling is essential for endothelial tube formation during vasculogenesis [J]. Development, 2004, 131(17): 4371–4380.

    Article  Google Scholar 

  27. LIU Y N, PATERSON M, BAUMGARDT S L, et al. Vascular endothelial growth factor regulation of endothelial nitric oxide synthase phosphorylation is involved in isoflurane cardiac preconditioning [J]. Cardiovascular Research, 2019, 115: 168–178.

    Article  Google Scholar 

Download references

Acknowledgement

The authors thank Dr. FENG Chuanliang and his team from the School of Materials Science and Engineering of Shanghai Jiao Tong University for providing the equipment and materials for hydrogel preparation.

Author information

Authors and Affiliations

  1. Department of Orthopedics, the Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, 200233, China

    Weijie Cai  (蔡伟杰), Musha Hamushan  (木沙•哈木山), Pengfei Cheng  (程鹏飞), Wanrun Zhong  (钟万润) & Pei Han  (韩培)

  2. School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China

    Changli Zhao  (赵常利)

Authors
  1. Weijie Cai  (蔡伟杰)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Musha Hamushan  (木沙•哈木山)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Changli Zhao  (赵常利)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pengfei Cheng  (程鹏飞)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wanrun Zhong  (钟万润)
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Pei Han  (韩培)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Wanrun Zhong  (钟万润) or Pei Han  (韩培).

Additional information

Foundation item

the National Natural Science Foundation of China (No. 81902210), and the Transnational Medicine Foundation of Shanghai Jiao Tong University (No. ZH2018QNA12)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Cai, W., Hamushan, M., Zhao, C. et al. Influence of Supramolecular Chiral Hydrogel on Cellular Behavior of Endothelial Cells Under High-Glucose-Induced Injury. J. Shanghai Jiaotong Univ. (Sci.) 26, 17–24 (2021). https://doi.org/10.1007/s12204-021-2256-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12204-021-2256-x

Key words

CLC number

Document code

Search

Navigation