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Comparison of osteogenesis between two kinds of stem cells from goat combined calcium phosphate cement in tissue engineering

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Abstract

To explore the possible mechanism of osteogenesis for deciduous teeth stem cells (DTSCs) in vivo/ vitro, stem cells from goat deciduous teeth (SGDs) were firstly isolated, induced and transplanted into immunocompromised mice. The SGDs’s mineralization pattern and osteogenesis were compared with bone marrow messenchymal stem cells (BMMSCs) from goats. SGDs have similar osteogenic differentiation pattern in vitro and bone-like tissue formation mechanism in vivo to BMMSCs; moreover SGDs have stronger alkaline phosphatase (ALP) gene expression and osteopontin (OPN) gene expression levels than BMMSCs; also SGDs can form more bone-like tissues than BMMSCs when cell-scaffold compounds are transplanted into immunocompromised mice. This pre-clinical study in a large-animal model confirms that DTSCs may be an appropriate source of stem cells in repairing bone defects with tissue engineering.

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Authors and Affiliations

  1. Department of Stomatology, the Sixth People’s Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233, China

    Wei Zhao  (赵 伟), Jia-yu Lu  (陆家瑜), Yong-ming Hao  (郝永明), Xiao-hui Qu  (瞿晓辉), Li Hua  (华 丽), Chunhua Cao  (曹春花) & De-rong Zou  (邹德荣)

  2. Oral Bioengineering Laboratory/Oral Tissue Engineering Laboratory, Shanghai Research Institute of Stomatology, the Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200011, China

    Xiu-li Zhang  (张秀丽)

Authors
  1. Wei Zhao  (赵 伟)
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  2. Jia-yu Lu  (陆家瑜)
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  3. Yong-ming Hao  (郝永明)
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  4. Xiu-li Zhang  (张秀丽)
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  5. Xiao-hui Qu  (瞿晓辉)
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  6. Li Hua  (华 丽)
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  7. Chunhua Cao  (曹春花)
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  8. De-rong Zou  (邹德荣)
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Corresponding author

Correspondence to De-rong Zou  (邹德荣).

Additional information

Foundation item: the Science and Technology Commission fund of Shanghai Municipality (No. 09JC1411700), the Collaboration Projects of Development and Research from Basic Science of Stomatology of Shanghai (No. S30206-KF09), and the Fund of Shanghai Jiaotong University School of Medicine (No. 09XJ21030)

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Zhao, W., Lu, Jy., Hao, Ym. et al. Comparison of osteogenesis between two kinds of stem cells from goat combined calcium phosphate cement in tissue engineering. J. Shanghai Jiaotong Univ. (Sci.) 16, 628–635 (2011). https://doi.org/10.1007/s12204-011-1200-x

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  • DOI: https://doi.org/10.1007/s12204-011-1200-x

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