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Excitation-emission matrices (EEMs) of colorectal tumors — tool for spectroscopic diagnostics of gastrointestinal neoplasia

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Abstract

The autofluorescence spectroscopy of biological tissues is a powerful tool for non-invasive detection of tissue pathologies and evaluation of any biochemical and morphological changes arising during the lesions’ growth. To obtain a full picture of the whole set of endogenous fluorophores appearing in the gastrointestinal (GI) tumors investigated, the technique of excitation-emission matrix (EEM) development was applied in a broad spectral region, covering the ultraviolet and visible spectral ranges. We could thus address a set of diagnostically-important chromophores and their alterations during tumor development, namely, collagen, elastin, nicotinamide adenine dinucleotide (NADH), flavins, porphyrins, while hemoglobin’s absorption influence on the spectra obtained could be evaluated as well. Comparisons are presented between EEM data of normal mucosae, benign polyps and malignant carcinoma, and the origins are determined of the fluorescence signals forming these matrices.

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Acknowledgements

This work was supported by the program for support of young scientists of the Bulgarian Academy of Sciences under Grant # DFNP-183-A1 “Macroscopic and microscopic fluorescence spectroscopy for gastrointestinal tumours investigations.”

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Authors and Affiliations

  1. Institute of Electronics, Bulgarian Academy of Sciences, Tsarigradsko Chaussee 72, Sofia, 1784, Bulgaria

    E. Borisova & Ts. Genova

  2. Saratov State University, Astrakhanskaya Str. 83, Saratov, 410012, Russia

    O. Semyachkina-Glushkovskaya

  3. Tsaritsa Yoanna — ISUL University Hospital, 8, Byalo More Str., Sofia, 1572, Bulgaria

    N. Penkov, I. Terziev & B. Vladimirov

Authors
  1. E. Borisova
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  2. Ts. Genova
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  3. O. Semyachkina-Glushkovskaya
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  4. N. Penkov
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  5. I. Terziev
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  6. B. Vladimirov
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Corresponding author

Correspondence to E. Borisova.

Additional information

Assoc. Prof. Dr. Ekaterina G. Borisova received the M. S. degree in Medical Physics and in Laser Physics from Physics Department of Sofia University, Bulgaria, in 2000. The Ph.D. degree on Physics of wave processes was received in Institute of Electronics, Bulgarian Academy of Sciences, Sofia, Bulgaria in 2005.

From 2004 to 2007, she was a Research Assistant with the laboratory of Condensed Matter Lasers in the same institute, where in the end of 2007 received Associated Professor position in the newly established Biophotonics laboratory. From 2012, she is a Scientific Secretary of the Institute of Electronics, Bulgarian Academy of Sciences, and from 2014 — Member of the Academic Council of Education Center of the Bulgarian Academy of Sciences. She is a co-author of six book chapters, more than 120 articles and conference full texts reports, as well as of five national and European patents in the field of biophotonics. Her recent investigations cover the field of molecular spectroscopy of biological samples, optical biopsy of human tissues and monitoring and detection of their pathological changes, as well as the problems of photodiagnosis and photodynamic therapy applications. Her researches in the field of optical spectroscopy of human tissues for oncologic applications were honored with the BAS Prize for best young scientist in the field of Physics (2004), Prize “Pythagoras” of the Ministry of Education and Sciences for the Best Young Scientist in Bulgaria in 2012, as well as with an UNESCO/L’Oreal fellowship “For Women in Science” for Bulgaria in 2014. From 2015, E. Borisova became a Senior SPIE Member, for her work in the field of biophotonics and applications.

Tsanislava Genova is a Ph.D. student at Institute of Electronics, Bulgarian Academy of Sciences. Both her B. S. and M. S. degrees are in Medical Physics at Faculty of Physics, University of Sofia “St. Kliment Ohridski”. Her research interests are in the field of biophotonics, particularly fluorescence diagnostic of gastrointestinal lesions, which is also the subject of her Ph. D. thesis. She is a co-author of eight articles. Her work was awarded for the best poster prize in the IV National Students Conference on Physics and Engineer Technologies — Plovdiv 2015, Plovdiv, Bulgaria and for the best poster award 2nd prize supported by SPIE at the 19th International Conference and School on Quantum Electronics "Laser Physics and Applications”, 2016.

Oxana Semyachkina-Glushkovskaya is professor and head of chair of Physiology of Human and Animals in Saratov State University, Russia. Her research interests are focused on the study of nature of stress and development of stress-related vascular catastrophes in the brain and the application in practical medicine the novel optical and mathematical technologies for prognosis and treatment of social important stress-induced diseases.

Assoc. Prof. Nikolai Penkov, M.D., Ph. D., has graduated Medical University Pleven, Bulgaria and finished specialization in surgery. Scince 2005 he has been a head of Coloproctology department in Clinic of Surgery, University Hospital “Tsaritsa Yoanna-ISUL”. His research interests are in hepatobiliary-pancreato area surgery, coloproctology and stomach surgery.

Dr. Ivan Terziev, M.D., is a specialist in general and clinical pathology and the Head of Department of Pathology in University Hospital “Tsaritsa Yoanna-ISUL”, since 2011. Dr. Ivan Terziev has achieved his higher medical education in Medical Academy, Sofia in 1987. In 1992, he acquired specialty degree for “Pathological anatomy and cytopathology”. His career starts as superintendent in Department of Anatomical Pathology, District Hospital, Blagoevgrad. Through 1988–2011, Dr. Ivan Terziev has been assistant, senior and chief assistant in University Hospital “Tsaritsa Yoanna-ISUL”. He is member of Bulgarian Society of Pathology and European Society of Neuropathology. His primary research interests are in the field of oncologic pathology. Dr. Ivan Terziev is co-author of more than 40 scientific papers and conference full text reports.

Prof. B. Vladimirov, M.D., Ph.D., has graduated Medical University, Sofia, Bulgaria. In 1994, he won the national prize for best physician in Bulgaria. A member of Bulgarian Society of Gastroenterology, Gastrointestinal Endoscopy and Abdominal Ultrasound- Chairman of the Department of Endoscopy, Bulgarian Association of Surgeons and Gastroenterologists, Bulgarian Association of Gastrointestinal Endoscopy — Chairman, Union of Scientists in Bulgaria, World Organization for Special Study of Oesophageal Diseases, European Society of Gastrointestinal Endoscopy, European Association for the Study of the Liver and World Organization of Gastroenterology. He is the head of Clinic of Gastroenterology, University Hospital “Tsaritsa Yoanna-ISUL”. His research interests are in gastrointestinal diagnostic and therapeutic endoscopy, peptic diseases, infection by Helicobacter pylori, cancer and other diseases of the digestive system.

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Borisova, E., Genova, T., Semyachkina-Glushkovskaya, O. et al. Excitation-emission matrices (EEMs) of colorectal tumors — tool for spectroscopic diagnostics of gastrointestinal neoplasia. Front. Optoelectron. 10, 292–298 (2017). https://doi.org/10.1007/s12200-017-0720-x

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  • DOI: https://doi.org/10.1007/s12200-017-0720-x

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