Histogram analysis of laser speckle contrast image for cerebral blood flow monitoring

Abstract

Laser speckle contrast imaging (LSCI) is a powerful tool for blood flow mapping. In this paper, we described a simple algorithm based on histogram analysis of laser speckle contrast image to provide rapid differentiation between macro- and microcirculations. The algorithm was successfully verified by the study of blood flow in rat cortex under functional activation.

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Correspondence to Arkady S. Abdurashitov.

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Arkady S. Abdurashitov graduated from high school №3 of Saratov. In present time, he is a student of National Research Saratov State University. He is the winner of the Student Scientific Conference, National Research Saratov State University, 2014 and participated in the poster session of Saratov Fall Meeting 2014 conference. His fields of interest are optics, physics, programming, data processing, data visualization.

Vladislav V. Lychagov received his M.S. degree in Physics in 2003 and Ph.D. degree in Optics in 2007, both from Saratov State University, Russia. Since that time, he obtained his postdoctoral training in Optics and Biophotonics department of Saratov State University. Since 2010, he is research associate professor of Optics and Biophotonics department. His research interests include wave and coherent optics, coherent imaging and microscopy, interferometric measurements and data processing.

Olga A. Sindeeva received her M.S. degree in Biology (diploma with excellence) in 2012 from Saratov State University, Russia. Since that time, she is a Ph.D. student in Saratov State University, Biological department, Chair of Physiology Human and Animals. Since 2014, she is research associate of Biological department. Her research interests include stress-induced vascular diseases, such as: stroke at different ages and sexes, hypertension, gastric ulcer; stress-limiting system, early indicators of vascular complications.

Oxana V. Semaychkina-Glushkovskaya received her M.Sc. equivalent in Physiology (diploma with excellence) in 1999, and Ph. D. equivalent in Biological Sciences in 2002, both from Saratov State University, Russia. From 2002 to 2013, she was an Assistant of Professor (2002–2007), Associate Professor (2007–2012), Professor (2012–2013) in Saratov State University, Department of Biology, Chair of Physiology Human and Animals. Since 2013, she is Head of Chair of Physiology of Human and Animals, Department of Biology, Saratov State University. Her research interests include stress-induced vascular damages, stress-limiting system, early indicators of vascular complications, mechanisms underlying neonatal stroke, transformation of peptic ulcer to cancer, role of sex hormones in stress-resistance and stress-reactivity of vascular system, development new animal models: stress-induced neonatal stroke, stress-related peptic ulcer and gastric cancer, technologies for a prognosis of stress-related vascular “catastrophes” such as stroke and ulcer bleeding.

Valery V. Tuchin received a M.S. degree in Radio-Physics and Electronics (1966), a Ph. D. degree in Optics (1974), and a DrSc in Laser Physics (1982) from Saratov State University, Saratov, Russia. Currently, he is a Professor and holds the Chair of Optics and Biophotonics of Saratov State University. He is also a Director of the Research-Educational Institute of Optics and Biophotonics at Saratov State University and Head of Laboratory on Laser Diagnostics of Technical and Living Systems, Inst. of Precise Mechanics and Control, RAS. His research interests include biophotonics, tissue optics, laser medicine, tissue optical clearing, and nanobiophotonics. He has authored more than 350 peer-reviewed papers, handbooks, monographs, text books, tutorials, and book chapters, holder of more than 50 patents. He is a member of SPIE, OSA, and IEEE. He is a fellow of SPIE and has been awarded Honored Science Worker of the Russia (1999), SPIE Educator Award (2007), FiDiPro (Finland) (2011), and Chime Bell Prize of Hubei Province, China (2014).

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Abdurashitov, A.S., Lychagov, V.V., Sindeeva, O.A. et al. Histogram analysis of laser speckle contrast image for cerebral blood flow monitoring. Front. Optoelectron. 8, 187–194 (2015). https://doi.org/10.1007/s12200-015-0493-z

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Keywords

  • laser speckle contrast imaging (LSCI)
  • histogram analysis
  • cerebral blood flow (CBF)
  • rat