Environmental Health and Preventive Medicine

, Volume 14, Issue 6, pp 328–335

Association between bone mineral density and lifestyle factors or vitamin D receptor gene polymorphism in adult male workers: a cross-sectional study

Regular Article

DOI: 10.1007/s12199-009-0104-y

Cite this article as:
Funakoshi, Y., Omori, H. & Katoh, T. Environ Health Prev Med (2009) 14: 328. doi:10.1007/s12199-009-0104-y



The aim of this cross-sectional study was to investigate the association between bone mineral density (BMD) and lifestyle factors, as well as the influence of vitamin D receptor (VDR) gene polymorphism, in adult male workers.


The subjects were 524 male employees aged 23–49 years (37.3 ± 5.4 years, mean ± standard deviation) working at a large-scale integrated manufacturing facility in Japan. BMD was measured at the nondominant radius by dual-energy X-ray absorptiometry. Lifestyle information was obtained by a questionnaire at the same time, and genomic DNA was isolated from peripheral leukocytes.


The genotype frequencies of VDR gene polymorphism detected by Taq I digestion were 81.3%, 17.9%, and 0.8% for TT, Tt, and tt, respectively. BMD was 0.56 ± 0.06 g/cm2. Analysis of covariance with adjustment for age and body mass index (BMI) revealed that subjects who had a past history of exercise, current exercise from 3 to 7 days a week or daily alcohol intake showed significantly higher BMD than subjects without these features (0.56 ± 0.06 versus 0.54 ± 0.06, 0.58 ± 0.06 versus 0.55 ± 0.06, and 0.57 ± 0.06 versus 0.55 ± 0.06, respectively) (P < 0.05). Subjects who ate only 2 meals a day or smoked ≥21 cigarettes a day showed significantly lower BMD if they had the Tt or tt genotype than if they had the TT genotype (0.51 ± 0.04 versus 0.56 ± 0.06 and 0.51 ± 0.05 versus 0.57 ± 0.06, respectively) (P < 0.05).


These findings suggest that the influence of lifestyle on BMD differs according to VDR gene polymorphism in adult male workers.


Bone mineral density Gene polymorphism Lifestyle Vitamin D receptor 

Copyright information

© The Japanese Society for Hygiene 2009

Authors and Affiliations

  • Yayoi Funakoshi
    • 1
  • Hisamitsu Omori
    • 1
  • Takahiko Katoh
    • 1
  1. 1.Department of Public Health, Graduate School of Medical SciencesKumamoto UniversityKumamotoJapan

Personalised recommendations