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LINC00852 Regulates Cell Proliferation, Invasion, Migration and Apoptosis in Hepatocellular Carcinoma Via the miR-625/E2F1 Axis

Abstract

Objective

This study investigates the function and regulatory mechanism of lncRNA LINC00852 in hepatocellular carcinoma (HCC) cells.

Methods

The effects of LINC00852 and E2F1 on HCC cell proliferation, invasion, migration and apoptosis were measured by MTT assay, Transwell invasion and migration assays and TUNEL staining, respectively. The apoptosis of HCC cells was further determined by the expression levels of apoptosis-related proteins (Bax and Bcl2). Dual-luciferase reporter assays verified the targeting relationships among LINC00852, miR-625 and E2F1.

Results

Overexpression of LINC00852 was positively associated with HCC cell proliferation, invasion and migration while negatively associated with the cell apoptosis. LINC00852 bound miR-625 which further targeted E2F1. Overexpressing miR-625 or down-regulating E2F1 reversed the oncogenic effects of LINC00852.

Conclusion

LINC00852 regulates HCC cell activities via the miR-625/E2F1 axis.

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Author Contributions

CS conceived the ideas; designed the experiments; performed the experiments; analyzed the data; provided critical materials; wrote the manuscript; supervised the study.All the authors have read and approved the final version for publication.

Funding

This research didn’t receive any funding.

Conflict of interest

The authors report no relationships that could be construed as a conflict of interest.

Ethics Approval and Consent to Participate

Experiments of this study were approved by the ethics committee of Hunan Cancer Hospital.

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Correspondence to Shi Chen.

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Chen, S. LINC00852 Regulates Cell Proliferation, Invasion, Migration and Apoptosis in Hepatocellular Carcinoma Via the miR-625/E2F1 Axis. Cel. Mol. Bioeng. 15, 207–217 (2022). https://doi.org/10.1007/s12195-021-00714-8

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  • DOI: https://doi.org/10.1007/s12195-021-00714-8

Keywords

  • Hepatocellular carcinoma
  • LINC00852
  • miR-625
  • E2F1
  • Proliferation
  • Invasion
  • Migration