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LINC00852 Regulates Cell Proliferation, Invasion, Migration and Apoptosis in Hepatocellular Carcinoma Via the miR-625/E2F1 Axis

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Abstract

Objective

This study investigates the function and regulatory mechanism of lncRNA LINC00852 in hepatocellular carcinoma (HCC) cells.

Methods

The effects of LINC00852 and E2F1 on HCC cell proliferation, invasion, migration and apoptosis were measured by MTT assay, Transwell invasion and migration assays and TUNEL staining, respectively. The apoptosis of HCC cells was further determined by the expression levels of apoptosis-related proteins (Bax and Bcl2). Dual-luciferase reporter assays verified the targeting relationships among LINC00852, miR-625 and E2F1.

Results

Overexpression of LINC00852 was positively associated with HCC cell proliferation, invasion and migration while negatively associated with the cell apoptosis. LINC00852 bound miR-625 which further targeted E2F1. Overexpressing miR-625 or down-regulating E2F1 reversed the oncogenic effects of LINC00852.

Conclusion

LINC00852 regulates HCC cell activities via the miR-625/E2F1 axis.

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References

  1. Beermann, J., M. T. Piccoli, J. Viereck, and T. Thum. Non-coding RNAs in development and disease: Background, mechanisms, and therapeutic approaches. Physiol Rev. 96:1297–1325, 2016.

    Article  Google Scholar 

  2. Burja, B., et al. Olive leaf extract attenuates inflammatory activation and DNA damage in human arterial endothelial cells. Front Cardiovasc Med. 6:56, 2019.

    Article  Google Scholar 

  3. Castelli, G., E. Pelosi, and U. Testa. Liver cancer: molecular characterization, clonal evolution and cancer stem cells. Cancers (Basel). 9:127, 2017.

    Article  Google Scholar 

  4. Chen, H. Z., S. Y. Tsai, and G. Leone. Emerging roles of E2Fs in cancer: an exit from cell cycle control. Nat Rev Cancer. 9:785–797, 2009.

    Article  Google Scholar 

  5. De Stefano, F., et al. Novel biomarkers in hepatocellular carcinoma. Dig. Liver Dis. 50:1115–1123, 2018.

    Article  Google Scholar 

  6. Du, W. W., et al. A circular RNA circ-DNMT1 enhances breast cancer progression by activating autophagy. Oncogene. 37:5829–5842, 2018.

    Article  Google Scholar 

  7. Ertosun, M. G., F. Z. Hapil, and O. Osman-Nidai. E2F1 transcription factor and its impact on growth factor and cytokine signaling. Cytokine Growth Factor Rev. 31:17–25, 2016.

    Article  Google Scholar 

  8. Fang, W., et al. microRNA-625 inhibits tumorigenicity by suppressing proliferation, migration and invasion in malignant melanoma. Oncotarget. 8:13253–13263, 2017.

    Article  Google Scholar 

  9. Fang, L., D. Kong, and W. Xu. MicroRNA-625-3p promotes the proliferation, migration and invasion of thyroid cancer cells by up-regulating astrocyte elevated gene 1. Biomed Pharmacother. 102:203–211, 2018.

    Article  Google Scholar 

  10. Farra, R., et al. The role of the transcription factor E2F1 in hepatocellular carcinoma. Curr Drug Deliv. 14:272–281, 2017.

    Google Scholar 

  11. Forner, A., M. Reig, and J. Bruix. Hepatocellular carcinoma. Lancet. 391:1301–1314, 2018.

    Article  Google Scholar 

  12. Global Burden of Disease Liver Cancer Collaboration, et al. The burden of primary liver cancer and underlying etiologies from 1990 to 2015 at the global, regional, and national level: results from the global burden of disease study 2015. JAMA Oncol. 3(1683–1691):2017, 1990.

    Google Scholar 

  13. Gong, X., B. Xu, L. Zi, and X. Chen. miR-625 reverses multidrug resistance in gastric cancer cells by directly targeting ALDH1A1. Cancer Manag Res. 11:6615–6624, 2019.

    Article  Google Scholar 

  14. Gu, Y., et al. SET7/9 promotes hepatocellular carcinoma progression through regulation of E2F1. Oncol Rep. 40:1863–1874, 2018.

    Google Scholar 

  15. Hollern, D. P., et al. E2F1 drives breast cancer metastasis by regulating the target gene fgf13 and altering cell migration. Sci Rep. 9:10718, 2019.

    Article  Google Scholar 

  16. Jin, L., C. Li, T. Liu, and L. Wang. A potential prognostic prediction model of colon adenocarcinoma with recurrence based on prognostic lncRNA signatures. Hum Genomics. 14:24, 2020.

    Article  Google Scholar 

  17. Li, Q., et al. Exosome-transmitted linc00852 associated with receptor tyrosine kinase AXL dysregulates the proliferation and invasion of osteosarcoma. Cancer Med. 9:6354–6366, 2020.

    Article  Google Scholar 

  18. Liu, P., et al. LINC00852 promotes lung adenocarcinoma spinal metastasis by targeting S100A9. J Cancer. 9:4139–4149, 2018.

    Article  Google Scholar 

  19. Pan, W., et al. lncRNA-PDPK2P promotes hepatocellular carcinoma progression through the PDK1/AKT/Caspase 3 pathway. Mol Oncol. 13:2246–2258, 2019.

    Article  Google Scholar 

  20. Schmitt, A. M., and H. Y. Chang. Long noncoding RNAs in cancer pathways. Cancer Cell. 29:452–463, 2016.

    Article  Google Scholar 

  21. Sia, D., A. Villanueva, S. L. Friedman, and J. M. Llovet. Liver cancer cell of origin, molecular class, and effects on patient prognosis. Gastroenterology. 152:745–761, 2017.

    Article  Google Scholar 

  22. Wang, Z., et al. miR-625 down-regulation promotes proliferation and invasion in esophageal cancer by targeting Sox2. FEBS Lett. 588:915–921, 2014.

    Article  Google Scholar 

  23. Wang, Y., et al. A novel lncRNA MCM3AP-AS1 promotes the growth of hepatocellular carcinoma by targeting miR-194-5p/FOXA1 axis. Mol Cancer. 18:28, 2019.

    Article  Google Scholar 

  24. Wang, L., et al. LINC00958 facilitates cervical cancer cell proliferation and metastasis by sponging miR-625-5p to upregulate LRRC8E expression. J Cell Biochem. 121:2500–2509, 2020.

    Article  Google Scholar 

  25. Wu, Z., S. Zheng, and Q. Yu. The E2F family and the role of E2F1 in apoptosis. Int J Biochem Cell Biol. 41:2389–2397, 2009.

    Article  Google Scholar 

  26. Xu, T. P., et al. E2F1 induces TINCR transcriptional activity and accelerates gastric cancer progression via activation of TINCR/STAU1/CDKN2B signaling axis. Cell Death Dis. 8:e2837, 2017.

    Article  Google Scholar 

  27. Zhang, Y., et al. Identification of potential prognostic long non-coding RNA biomarkers for predicting recurrence in patients with cervical cancer. Cancer Manag Res. 12:719–730, 2020.

    Article  Google Scholar 

  28. Zheng, H., et al. MiR-625-3p promotes cell migration and invasion via inhibition of SCAI in colorectal carcinoma cells. Oncotarget. 6:27805–27815, 2015.

    Article  Google Scholar 

  29. Zhou, X., et al. miR-625 suppresses tumour migration and invasion by targeting IGF2BP1 in hepatocellular carcinoma. Oncogene. 35:5078, 2016.

    Article  Google Scholar 

  30. Zhou, W. B., et al. miR-625 suppresses cell proliferation and migration by targeting HMGA1 in breast cancer. Biochem Biophys Res Commun. 470:838–844, 2016.

    Article  Google Scholar 

Download references

Author Contributions

CS conceived the ideas; designed the experiments; performed the experiments; analyzed the data; provided critical materials; wrote the manuscript; supervised the study.All the authors have read and approved the final version for publication.

Funding

This research didn’t receive any funding.

Conflict of interest

The authors report no relationships that could be construed as a conflict of interest.

Ethics Approval and Consent to Participate

Experiments of this study were approved by the ethics committee of Hunan Cancer Hospital.

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Authors and Affiliations

  1. Department of Pathology, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, No. 283, Tongzipo Road, Yuelu District, Changsha, 410013, Hunan, People’s Republic of China

    Shi Chen

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  1. Shi Chen
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Correspondence to Shi Chen.

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Associate Editor Michael R. King oversaw the review of this article.

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Chen, S. LINC00852 Regulates Cell Proliferation, Invasion, Migration and Apoptosis in Hepatocellular Carcinoma Via the miR-625/E2F1 Axis. Cel. Mol. Bioeng. 15, 207–217 (2022). https://doi.org/10.1007/s12195-021-00714-8

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  • DOI: https://doi.org/10.1007/s12195-021-00714-8

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