Stem cells offer tremendous promise for regenerative medicine as they can become a variety of cell types. They also continuously proliferate, providing a renewable source of cells. Recently, it has been found that 3D printing constructs using stem cells, can generate models representing healthy or diseased tissues, as well as substitutes for diseased and damaged tissues. Here, we review the current state of the field of 3D printing stem cell derived tissues. First, we cover 3D printing technologies and discuss the different types of stem cells used for tissue engineering applications. We then detail the properties required for the bioinks used when printing viable tissues from stem cells. We give relevant examples of such bioprinted tissues, including adipose tissue, blood vessels, bone, cardiac tissue, cartilage, heart valves, liver, muscle, neural tissue, and pancreas. Finally, we provide future directions for improving the current technologies, along with areas of focus for future work to translate these exciting technologies into clinical applications.
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The Willerth lab would like to acknowledge funding from the Stem Cell Network, the Canada Research Chairs program, the NSERC Discovery Grant program, MITACS, and the British Columbia Innovation Council. Dr. Willerth has a collaborative research agreement with Aspect Biosystems. Laura De la vega has received graduate support from MITACS. Dr. Joddar acknowledges NIH BUILD Pilot 8UL1GM118970-02, NIH 1SC2HL134642-01 and the NSF-PREM program (DMR 1205302). Nishat Tasnim acknowledges the Anita Mochen Loya fellowship at UTEP. Matthew Alonzo acknowledges the Eloise E. and Patrick B. Wieland fellowship at UTEP. Schweta Anil Kumar, Laila Abelseth, and Meitham Amereh have no other funding sources to declare outside of those mentioned earlier.
This article does not contain any studies with human participants or animals performed by any of the authors.
Associate Editor Michael R. King oversaw the review of this article.
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Tasnim, N., De la Vega, L., Anil Kumar, S. et al. 3D Bioprinting Stem Cell Derived Tissues. Cel. Mol. Bioeng. 11, 219–240 (2018). https://doi.org/10.1007/s12195-018-0530-2
- Pluripotent stem cells
- Regenerative medicine
- Tissue engineering
- Drug delivery
- Stem cell niche