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Viscoelasticity Measurements Reveal Rheological Differences Between Stem-like and Non-stem-like Breast Cancer Cells

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Abstract

Defining the characteristics of cancer stem cells (CSC) has become an important subject in cancer research during the past decade. Although molecular surface expression levels have been used for CSC recognition, the clinical and prognostic impacts of these markers have remained a controversial issue. The finding that cancerous cells are considerably more deformable than normal ones provides the motivation for the hypothesis that the mechanical properties can be used as biomarkers to distinguish between stem-like and non-stem-like cancer cells. In this study, using micropipette aspiration (MA) and intracellular particle tracking (IPT) microrheology, measurements of the whole-cell and local viscoelasticity were made on four breast cancer cell lines with different CSC phenotypes based on their surface markers. Stem-like Hs578T and MDA-MB-231 cell lines were found to be the most deformable, while the non-stem-like MDA-MB-468 line was the least deformable. The non-stem-like BT-20 cell line showed an intermediate deformability. The enhanced deformability for stem-like cells was consistent with the observed lower and more dispersed F-actin content for the stem-like cells. Therefore, the cytoskeleton-related differences in the rheological properties of cancer cells can be a potential biomarker for CSC and eventually lead to novel cancer diagnostic and therapeutic methods.

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Acknowledgments

This work was supported by National Science Foundation grants CBET-1106118 and Major Research Instrumentation CBET-1039869. The authors would like to thank Mr. Grady Carlson (Department of Chemical and Biomolecular Engineering, Ohio University) for his collaboration and help in lab instruction.

Conflict of interest

Amina Mohammadalipour, Monica Burdick and David Tees declare that they have no conflict of interest.

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No human studies or animal studies were carried out by the authors for this article.

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Authors and Affiliations

  1. Department of Physics and Astronomy, Ohio University, Athens, OH, 45701, USA

    A. Mohammadalipour & D. F. J. Tees

  2. Department of Chemical and Biomolecular Engineering, Ohio University, Athens, OH, 45701, USA

    A. Mohammadalipour & M. M. Burdick

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  1. A. Mohammadalipour
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Correspondence to D. F. J. Tees.

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Associate Editor Michael R. King oversaw the review of this article.

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Mohammadalipour, A., Burdick, M.M. & Tees, D.F.J. Viscoelasticity Measurements Reveal Rheological Differences Between Stem-like and Non-stem-like Breast Cancer Cells. Cel. Mol. Bioeng. 10, 235–248 (2017). https://doi.org/10.1007/s12195-017-0485-8

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