Cellular and Molecular Bioengineering

, Volume 7, Issue 3, pp 355–368

Shrink Wrapping Cells in a Defined Extracellular Matrix to Modulate the Chemo-Mechanical Microenvironment

  • Rachelle N. Palchesko
  • John M. Szymanski
  • Amrita Sahu
  • Adam W. Feinberg
Article

DOI: 10.1007/s12195-014-0348-5

Cite this article as:
Palchesko, R.N., Szymanski, J.M., Sahu, A. et al. Cel. Mol. Bioeng. (2014) 7: 355. doi:10.1007/s12195-014-0348-5

Abstract

Cell–matrix interactions are important for the physical integration of cells into tissues and the function of insoluble, mechanosensitive signaling networks. Studying these interactions in vitro can be difficult because the extracellular matrix (ECM) proteins that adsorb to in vitro cell culture surfaces do not fully recapitulate the ECM-dense basement membranes to which cells such as cardiomyocytes and endothelial cells adhere to in vivo. Towards addressing this limitation, we have developed a surface-initiated assembly process to engineer ECM proteins into nanostructured, microscale sheets that can be shrink wrapped around single cells and small cell ensembles to provide a functional and instructive matrix niche. Unlike current cell encapsulation technology using alginate, fibrin or other hydrogels, our engineered ECM is similar in density and thickness to native basal lamina and can be tailored in structure and composition using the proteins fibronectin, laminin, fibrinogen, and/or collagen type IV. A range of cells including C2C12 myoblasts, bovine corneal endothelial cells and cardiomyocytes survive the shrink wrapping process with high viability. Further, we demonstrate that, compared to non-encapsulated controls, the engineered ECM modulates cytoskeletal structure, stability of cell–matrix adhesions and cell behavior in 2D and 3D microenvironments.

Keywords

Fibronectin Laminin Collagen Type IV Fribrinogen Myocyte Encapsulation Surface-initiated assembly c2c12 

Supplementary material

12195_2014_348_MOESM1_ESM.mpg (3.4 mb)
Supplementary material 1 (MPG 3468 kb)
12195_2014_348_MOESM2_ESM.mpg (876 kb)
Supplementary material 2 (MPG 876 kb)

Copyright information

© Biomedical Engineering Society 2014

Authors and Affiliations

  • Rachelle N. Palchesko
    • 1
  • John M. Szymanski
    • 1
  • Amrita Sahu
    • 1
  • Adam W. Feinberg
    • 1
    • 2
  1. 1.Department of Biomedical EngineeringCarnegie Mellon UniversityPittsburghUSA
  2. 2.Department of Materials Science and EngineeringCarnegie Mellon UniversityPittsburghUSA

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