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Landing Rate Measurements to Detect Fibrinogen Adsorption to Non-fouling Surfaces

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Abstract

Rapid advances in non-fouling surface technology have pushed the performance of novel coatings toward the detection limit of established protein density quantification techniques. Hence, there is an urgent need for more sensitive detection strategies. Previously we demonstrated that landing rate measurements of microtubules can reveal kinesin surface coverages between 0.1 and 10 μm−2. In this report, we quantify the binding kinetics of highly fluorescent markers to surface-adhered proteins and demonstrate the of protein surface densities in the range of 0.1–1000 μm−2. We utilize this technique to measure kinesin densities on casein-coated glass surfaces and fibrinogen densities on non-fouling polyethylene glycol methacrylate (PEGMA) surfaces. The use of nanospheres (i) potentially permits the detection of a variety of adsorbed proteins, (ii) facilitates the determination of the landing rate due to their uniformity, and (iii) extends the dynamic range of the method due to their small size.

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Acknowledgments

The authors thank Amit Singh of PERC, University of Florida, and Siheng He for helpful discussions. H.H. was supported by NSF Award DMR 1015486.

Conflict of interest

The authors do not have any conflict of interest to declare.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Columbia University, 351L Engineering Terrace MC 8904, 1210 Amsterdam Avenue, New York, NY, 10027, USA

    Ashutosh Agarwal & Henry Hess

  2. Department of Materials Science and Engineering, University of Florida, Gainesville, FL, 32611, USA

    Elizabeth Luria

  3. Department of Chemical Engineering, Department of Materials Engineering, and Macromolecular Science and Engineering Program, University of Michigan, Ann Arbor, MI, 48109, USA

    Xiaopei Deng & Joerg Lahann

Authors
  1. Ashutosh Agarwal
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  2. Elizabeth Luria
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  3. Xiaopei Deng
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  4. Joerg Lahann
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  5. Henry Hess
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Correspondence to Henry Hess.

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Associate Editor David Sept oversaw the review of this article.

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Agarwal, A., Luria, E., Deng, X. et al. Landing Rate Measurements to Detect Fibrinogen Adsorption to Non-fouling Surfaces. Cel. Mol. Bioeng. 5, 320–326 (2012). https://doi.org/10.1007/s12195-012-0239-6

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  • DOI: https://doi.org/10.1007/s12195-012-0239-6

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