Cellular and Molecular Bioengineering

, Volume 5, Issue 2, pp 155–164

Mechanical Tension Modulates Local and Global Vesicle Dynamics in Neurons

  • W. W. Ahmed
  • T. C. Li
  • S. S. Rubakhin
  • A. Chiba
  • J. V. Sweedler
  • T. A. Saif
BMES 2011 Outstanding Papers

DOI: 10.1007/s12195-012-0223-1

Cite this article as:
Ahmed, W.W., Li, T.C., Rubakhin, S.S. et al. Cel. Mol. Bioeng. (2012) 5: 155. doi:10.1007/s12195-012-0223-1

Abstract

Growing experimental evidence suggests that mechanical tension plays a significant role in determining the growth, guidance, and function of neurons. Mechanical tension in axons contributes to neurotransmitter clustering at the Drosophila neuromuscular junction (NMJ) and is actively regulated by neurons both in vitro and in vivo. In this work, we applied mechanical strain on in vivoDrosophila neurons and in vitroAplysia neurons and studied their vesicle dynamics by live-imaging. Our experiments show that mechanical stretch modulates the dynamics of vesicles in two different model systems: (1) The global accumulation of synaptic vesicles (SV) at the Drosophila NMJ and (2) the local motion of individual large dense core vesicles (LDCV) in Aplysia neurites. Specifically, a sustained stretch results in enhanced SV accumulation in the Drosophila NMJ. This increased SV accumulation occurs in the absence of extracellular Ca2+, plateaus after approximately 50 min, and persists for at least 30 min after stretch is reduced. On the other hand, mechanical compression in Aplysia neurites immediately disrupts LDCV motion, leading to decreased range and processivity. This impairment of LDCV motion persists for at least 15 min after tension is restored. These results show that mechanical stretch modulates both local and global vesicle dynamics and strengthens the notion that tension serves a role in regulating neuronal function.

Keywords

Cell mechanics Subcellular Live-imaging Vesicle tracking Drosophila Aplysia 

Supplementary material

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Copyright information

© Biomedical Engineering Society 2012

Authors and Affiliations

  • W. W. Ahmed
    • 1
    • 2
  • T. C. Li
    • 3
  • S. S. Rubakhin
    • 2
    • 4
  • A. Chiba
    • 3
  • J. V. Sweedler
    • 2
    • 4
  • T. A. Saif
    • 1
    • 2
    • 5
  1. 1. Department of Mechanical Science and EngineeringUniversity of Illinois at Urbana-Champaign, 2101D Mechanical Engineering LaboratoryUrbanaUSA
  2. 2.Beckman Institute for Advanced Science and TechnologyUniversity of IllinoisUrbanaUSA
  3. 3.Department of BiologyUniversity of MiamiCoral GablesUSA
  4. 4.Department of ChemistryUniversity of IllinoisUrbanaUSA
  5. 5.Micro and Nanotechnology LaboratoryUniversity of Illinois at Urbana-Champaign, 2101D Mechanical Engineering LaboratoryUrbanaUSA

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