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Optimal Temperature for Hypothermia Intervention in Mouse Model of Skeletal Muscle Ischemia Reperfusion Injury

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Abstract

Ischemia–reperfusion (IR) in skeletal muscles is a prevalent problem associated with many diseases and injuries. It can result in loss of muscle mass and function, and in severe cases, amputation or even death. Hypothermia has been shown to be effective in protecting muscles from the IR injury, but the minimal effective hypothermic temperature has not been clearly defined. This is a problem because excessively low temperatures may have undesired pathophysiological consequences. Thus, in this study, we performed a series of experiments aimed at identifying the minimal hypothermic temperature that is needed for protection against IR injury. In the experiment, a pressure inflating cuff and hypothermia cuff were designed to provide more controllable ischemia and hypothermia. Our results indicate that a hypothermic temperature of 17 °C can completely protect skeletal muscle contractile function and morphology from the damaging effects of 1-h ischemia. Thus, hypothermic intervention may reach the maximum protective effects in the deep hypothermia range near 17 °C for skeletal muscles against 1-h IR injuries. The results suggest that hypothermic temperatures below 17 °C may not offer further benefits while risking pathophysiological complications.

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Acknowledgments

This article is a part of the celebration dedicated to Dr. Shu Chien’s 80th birthday, for his leadership, contribution and dedication to the field of biomechanics and bioengineering. It is his guidance, mentorship and continuous support that made this work possible. Thanks to Dr. Niren Angle for initiating the collaboration in ischemia–reperfusion with a different focus that opened the door to this study. We would also like to express our gratitude to Dr. Alan Hargens, Orthopaedic Surgery, UCSD for supplying the laser Doppler flow meter. We are grateful to Kersi Pestonjamasp, Moores Cancer Center, UCSD for the valuable help in microscopy.

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Authors and Affiliations

  1. Department of Bioengineering, University of California, San Diego, 9500 Gilman Dr. MC-0412, La Jolla, CA, 92037-0412, USA

    Dayu Teng & Troy A. Hornberger

  2. Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, Madison, 2015 Linden Drive, Madison, WI, 53706, USA

    Troy A. Hornberger

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  1. Dayu Teng
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  2. Troy A. Hornberger
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Correspondence to Dayu Teng.

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Associate Editor John Shyy & Yingxiao Wang oversaw the review of this article.

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Teng, D., Hornberger, T.A. Optimal Temperature for Hypothermia Intervention in Mouse Model of Skeletal Muscle Ischemia Reperfusion Injury. Cel. Mol. Bioeng. 4, 717–723 (2011). https://doi.org/10.1007/s12195-011-0206-7

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  • DOI: https://doi.org/10.1007/s12195-011-0206-7

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