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Single Molecule Detection of One, Two and Multiplex Proteins Involved in DNA/RNA Transaction

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Abstract

Cellular processes involve complex arrangement of proteins engaged in a multitude of reactions, yet in a highly coordinated manner. The level of complexity, however, makes it difficult to investigate the role played by the individual protein constituent. Data taken from the conventional bulk solution methods suffer from ensemble averaging effect in which information from individual molecules is masked. The single molecule detection method overcomes this limitation by offering unique tools for monitoring the activity of individual molecules in isolation and in real-time dynamics. Included in this review are recent articles of single molecule studies representing a diverse array of experimental platforms which demonstrate the power and spectrum of single molecule detection.

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Authors and Affiliations

  1. Bioengineering Department, University of Illinois Urbana-Champaign, Champaign, IL, USA

    Yupeng Qiu & Sua Myong

  2. Institute for Genomic Biology, University of Illinois Urbana-Champaign, Champaign, IL, USA

    Sua Myong

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  1. Yupeng Qiu
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  2. Sua Myong
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Correspondence to Sua Myong.

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Associate Editor Yingxiao Wang oversaw the review of this article.

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Qiu, Y., Myong, S. Single Molecule Detection of One, Two and Multiplex Proteins Involved in DNA/RNA Transaction. Cel. Mol. Bioeng. 4, 125–137 (2011). https://doi.org/10.1007/s12195-011-0159-x

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  • DOI: https://doi.org/10.1007/s12195-011-0159-x

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