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Cellular and Molecular Bioengineering

, Volume 3, Issue 4, pp 345–360 | Cite as

Determining Cell Fate Transition Probabilities to VEGF/Ang 1 Levels: Relating Computational Modeling to Microfluidic Angiogenesis Studies

  • Anusuya DasEmail author
  • Doug Lauffenburger
  • Harry Asada
  • Roger Kamm
Article

Abstract

Angiogenesis is crucial during many physiological processes, and is influenced by various biochemical and biomechanical factors. Two such factors: VEGF and Ang 1 are known to be critical and we demonstrate here their effect of sprout formation in an in vitro microfluidic system. Previously, we have developed a 3D hybrid, agent-field model where individual cells are modeled as sprout-forming agents in a matrix field. We have conducted microfluidic experiments under different concentrations of VEGF and Ang 1 and analyzed the difference in sprout number and sprout lengths using Decision Tree Analysis. We demonstrate that under specific transition probabilities, the model gives us capillary characteristics similar to those seen in experiments (R 2 ~ 0.82–0.99). Thus, this model can be used to cluster sprout morphology as a function of various influencing factors and, within bounds, predict if a certain growth factor will affect migration or proliferation as it impacts sprout morphology

Keywords

Angiogenesis Capillary characteristics Microfluidics 

Notes

Acknowledgments

We would like to thank Wahleed Farhat for designing the microfluidic device wafers. We would like to acknowledge NSF-EFRI grant# 0735997 and the Singapore-MIT Alliance for Research and Technology for funding.

Supplementary material

12195_2010_146_MOESM1_ESM.doc (762 kb)
Supplementary material 1 (DOC 762 kb)

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Copyright information

© Biomedical Engineering Society 2010

Authors and Affiliations

  • Anusuya Das
    • 1
    • 3
    Email author
  • Doug Lauffenburger
    • 1
  • Harry Asada
    • 2
  • Roger Kamm
    • 1
    • 2
  1. 1.Departments of Biological EngineeringMassachusetts Institute of TechnologyCambridgeUSA
  2. 2.Departments of Mechanical EngineeringMassachusetts Institute of TechnologyCambridgeUSA
  3. 3.Departments of Biological EngineeringMassachusetts Institute of TechnologyCambridgeUSA

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