Cellular and Molecular Bioengineering

, Volume 3, Issue 4, pp 369–375 | Cite as

Cyclic Strain Promotes Migration and Proliferation of Human Periodontal Ligament Cell via PI3K Signaling Pathway

  • Yue Han
  • Jinsong PanEmail author
  • Xiaoli Wang
  • Yingxin Qi
  • Shiwei Wang
  • Zhiqiang Yan


Mechanical stimulations have significant impact on cell growth and migration, while little is known on the mechanism of human periodontal ligament cells (hPDLCs) behavior alteration under cyclic strain application. To elucidate the role of PI3K/Akt signaling pathway in cyclic strain-induced migration and proliferation of hPDLCs, 10 or 20%-elongation of cyclic strain at 0.1 Hz-frequency was applied to hPDLCs for 6 h or 24 h with FX-4000T system. The expressions of MMP-13 and p-Akt were examined by Western blotting, and cell proliferation was also detected by BrdU assay. A significant enhance of hPDLCs migration was observed after 24 h of cyclic strain application evaluated with wound healing method, and 20% was found to be more sensitive than 10% of cyclic strain. The strain also significantly increased MMP-13 and p-Akt expressions in hPDLCs as compared with the static. More importantly, the cyclic strain-induced hPDLC migration was suppressed by PI3K/Akt inhibitor wortmannin, and blocked with MMP-13 RNAi pretreatment before loading of 10 or 20%-strain. Additionally, wortmannin suppressed not only the activation of p-Akt, MMP-13 and PCNA, but also the cell proliferation under cyclic strain application in hPDLCs. The findings suggest that cyclic strain promotes the migration and proliferation of hPDLCs by up-regulating the expressions of MMP-13 and PCNA through PI3K/Akt signaling pathway.


Cyclic strain hPDLCs Migration Proliferation PI3K signaling pathway 



We would like to thank prof. Zong-Lai Jiang at Institute of Mechanobiology & Medical Engineering, Shanghai Jiao Tong University, China for his valuable suggestions and scientific discussion. This research was supported by grants from the National Natural Science Foundation of China, No. 10502034.


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Copyright information

© Biomedical Engineering Society 2010

Authors and Affiliations

  • Yue Han
    • 2
  • Jinsong Pan
    • 1
    Email author
  • Xiaoli Wang
    • 1
  • Yingxin Qi
    • 2
  • Shiwei Wang
    • 1
  • Zhiqiang Yan
    • 2
  1. 1.Dental Department, Shanghai 1st People’s Hospital Affiliated to School of MedicineShanghai Jiao Tong UniversityHongkou, ShanghaiChina
  2. 2.Institute of Mechanobiology and Medical EngineeringShanghai Jiao Tong UniversityShanghaiChina

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