Abstract
It has been well recognized that bone adapts its structure to best meet its mechanical environment. However, the cellular mechanism underlying bone adaptation is not well understood. Significant research efforts have been made towards understanding the cellular mechanotransduction mechanism in bone by studying cells in monolayer. These in vitro studies have indeed shown that bone cells are mechanosensitive and contributed critical insights into the mechanism of transduction. However, the monolayer approach may be limited in that it does not reflect the interaction of the osteocyte with its extracellular environment. It is in this context, we developed a microchamber system to mimic the in vivo extracellular environment in bone. Specifically, the osteocyte process appears to have unique ultrastructural characteristics which are potentially the molecular sites of cellular mechanosensitivity. In this study we describe a microfluidics approach aimed at replicating the bone canalicular system both in terms of geometry and fluid flow driven by exogenous loading. We demonstrate that individual osteocytes can be successfully cultured in this system for extended periods and extend processes similar to those observed in vivo. Our hope is that this approach will allow the role of flow over the osteocyte process to be better delineated.
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References
Burger E. H. and J. Klein-Nulend. Mechanotransduction in bone—role of the lacuno-canalicular network. Faseb. J. 13:101–112, 1999
Burger E. H., J. Klein-Nulend, A. van der Plas, and P. J. Nijweide. Function of osteocytes in bone—their role in mechanotransduction. J. Nutr. 125:2020S–2023S, 1995
Cowin S. C., L. Moss-Salentijn, and M. L. Moss. Candidates for the mechanosensory system in bone. J. Biomech. Eng. 113:191–197, 1991
Frost H. M. Perspectives: bone’s mechanical usage windows. Bone Miner. 19:257–271, 1992
Hung C. T., S. R. Pollack, T. M. Reilly, C. T. Brighton. Real-time calcium response of cultured bone cells to fluid flow. Clin. Orth. Rel. Res. 313:256–269, 1995
Jacobs C. R., C. E. Yellowley, B. R. Davis, Z. Zhou, J. M. Cimbala and H. J. Donahue. Differential effect of steady versus oscillating flow on bone cells. J. Biomech. 31:969–976, 1998
Leclerc E., B. David, L. Griscom, B. Lepioufle, T. Fujii, P. Layrolle and C. Legallaisa. Study of osteoblastic cells in a microfluidic environment. Biomaterials 27:586–595, 2006
Weinbaum S., S. C. Cowin and Y. Zeng. A model for the excitation of osteocytes by mechanical loading-induced bone fluid shear stresses. J. Biomech. 27:339–360, 1994
Wolff J. (1892) Das Gesetz der Transformation der Knochen. A. Hirchwild., Berlin
You L., S. C. Cowin, M. B. Schaffler and S. Weinbaum. A model for strain amplification in the actin cytoskeleton of osteocytes due to fluid drag on pericellular matrix. J. Biomech. 34:1375–1386, 2001
You, J. S., M. M, Saunders, C. E. Yellowley, H. J. Donahue, and C. R. Jacobs. Oscillatory flow stimulates prostaglandin E2 release via protein Kinase A in MC3T3-E1 osteoblasts involving cyclooxegenase-2. Trans. 47th Orth. Res. Soc. 47:0326, 2001
You L. D., S. Weinbaum, S. C. Cowin and M. B. Schaffler. Ultrastructure of the osteocyte process and its pericellular matrix. Anat. Rec. 278A:505–513, 2004
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This wok was supported by NIH Grant AR45989.
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You, L., Temiyasathit, S., Tao, E. et al. 3D Microfluidic Approach to Mechanical Stimulation of Osteocyte Processes. Cel. Mol. Bioeng. 1, 103–107 (2008). https://doi.org/10.1007/s12195-008-0010-1
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DOI: https://doi.org/10.1007/s12195-008-0010-1