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Expression of heme oxygenase-1 in type II pneumocytes protects against heatstroke-induced lung damage

Cell Stress and Chaperones volume 26, pages 67–76 (2021)Cite this article

Abstract

Heatstroke (HS) is an acute clinical disease characterized by abnormal hyperthermia and multi-organ dysfunction. Heme oxygenase (HO)-1, also called heat shock protein (HSP)32, is induced by hyperthermia and also plays protective roles in many lung disease models. Based on this phenomenon, we investigated the protective role of endogenous HO-1 in heat-induced lung damage in rats. Male Sprague-Dawley (SD) rats were separated into three groups: (a) normothermic sham, (b) HS, and (c) SnPP (inhibitor of HO-1) pretreatment rats. In the HS group, rats were killed at various time points (1, 3, 6, and 12 h after heat exposure) in order to analyze messenger ribonucleic acid (mRNA) and protein levels. Lung sections were examined for tissue damage and localization of HO-1 using immunofluorescence double labeling. We found that HS induced lung pathology (congested and thickened lung septa). The level of HO-1 mRNA was increased at 1 h, and the protein level peaked at 6 h after heat exposure. Pretreatment with SnPP (tin-protoporphyrin IX, 30 mg/kg, intraperitoneal injection for 1 h before heat exposure) aggravated the lung damage. Furthermore, we demonstrated HO-1 expression in lung type II pneumocytes. Our results suggest that endogenous HO-1 is protective against HS-induced lung damage. Induction of HO-1 may be a potential therapeutic strategy for treating heat-related diseases.

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Funding

This work was supported by grants from the Tri-Service General Hospital Songsang Branch (TSGH Songsang Branch 10706) and Tri-Service General Hospital grant (TSGH-105-089).

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Author notes

  1. Chin-Kun Tseng and Tsung-Ta Liu contributed equally to this article and both qualify as first authors.

Affiliations

  1. Tri-Service General Hospital Songsang Branch, National Defense Medical Center, Taipei, Taiwan

    Chin-Kun Tseng

  2. Department Electronic Engineering, National Taipei University of Technology, Taipei, Taiwan

    Chin-Kun Tseng

  3. Department of Physiology and Biophysics, National Defense Medical Center, Taipei, Taiwan

    Tsung-Ta Liu

  4. Nursing Department, Center for General Education, Kang-Ning University, Tainan, Taiwan

    Tsung-Ta Liu

  5. Genomic Medicine Core Laboratory, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan

    Tsung-Chieh Lin

  6. Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, No.161, Sec. 6, Min-Chuan E. Rd., Neihu, 114, Taipei, Taiwan

    Chia-Pi Cheng

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  1. Chin-Kun Tseng
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  2. Tsung-Ta Liu
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  4. Chia-Pi Cheng
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Correspondence to Chia-Pi Cheng.

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Tseng, CK., Liu, TT., Lin, TC. et al. Expression of heme oxygenase-1 in type II pneumocytes protects against heatstroke-induced lung damage. Cell Stress and Chaperones 26, 67–76 (2021). https://doi.org/10.1007/s12192-020-01152-7

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  • DOI: https://doi.org/10.1007/s12192-020-01152-7

Keywords

  • Heatstroke
  • Heme oxygenase-1
  • SnPP
  • Type II pneumocyte