Abstract
Endemic arsenism is widely distributed in the world, which can damage multiple organs, especially in skin and liver. The etiology is clear, but the mechanisms involved remain unknown. Ubiquitin-proteasome pathway (UPP) is the main pathway regulating protein degradation of which proteasome subunit beta type-5(PSMB5) plays a dominant role. This paper aims to study the role and mechanism of PSMB5 in sodium arsenite (NaAsO2)–induced oxidative stress liver injury in L-02 cells. Firstly, L-02 cells were exposed to different concentrations of NaAsO2 to establish a liver injury model of oxidative stress, and then mechanisms of oxidative stress were studied with carbobenzoxyl-leucyl-leucl-leucll-line (MG132) and knockdown PSMB5 (PSMB5-siRNA). The oxidative stress indicators, levels of 20S proteasome, the transcription and protein expression levels of PSMB5, Cu-Zn superoxide dismutase (SOD1), and glutathione peroxidase 1 (GPx1) were detected. The results demonstrated that NaAsO2 could induce oxidative stress–induced liver injury and the activity of 20S proteasome and the protein expression of PSMB5, SOD1, and GPx1 decreased. After MG132 or PSMB5-siRNA pretreatment, the gene expression of PSMB decreased. After MG132 or PSMB5-siRNA pretreatment, and then L-02 cells were treated with NaAsO2, the gene expression of PSMB remarkably decreased; however, the protein expression of SOD1 and GPx1 increased. Overall, NaAsO2 exposure could induce oxidative stress liver injury and low expression of PSMB5 in L-02 cells, and PSMB5 might play an important role in the regulation of oxidative stress by regulating the expression of SOD1 and Gpx1.
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Funding
This work has been supported by the National Natural Science Funding of China (No. 81860561, 81430077), the Natural Science Foundation of Guizhou Province (No. [2016]1119, [2014]7097), and the first-class discipline construction project in Guizhou Province-Public Health and Preventive Medicine (No. 2017[85]).
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Aihua Zhang and Yong Hu conceived the idea of this experiment. Ying Lv, Hu Qian, Mingyang Shi, Wen wang, Yuancui Zheng, Zhong Yang, Liuyu Peng, and Dingnian Bi conducted experiments. Ying Lv analyzed data and performed statistical analysis. Ying Lv and Yong Hu wrote the manuscript. All authors had final approval of the submitted and published version.
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Lv, Y., Hu, Q., Shi, M. et al. The role of PSMB5 in sodium arsenite–induced oxidative stress in L-02 cells. Cell Stress and Chaperones 25, 533–540 (2020). https://doi.org/10.1007/s12192-020-01104-1
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DOI: https://doi.org/10.1007/s12192-020-01104-1