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Genetic profiling of Hsp70 gene in Murrah buffalo (Bubalus bubalis) under sub-tropical climate of India

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Cell Stress and Chaperones Aims and scope

Abstract

This study was aimed to genetic profiling of heat shock protein 70 (Hsp70) gene in Murrah buffalo investigating 50 unrelated adult animals at ICAR-Research Complex for Eastern Region, Patna (India) in winter, spring, and summer. PCR ready genomic DNA samples and season-wise total RNA samples were prepared. The PCR products of Hsp70 eluted from agarose gel were sequenced and analyzed. The first-strand cDNA was synthesized and concentration was equalized to 25 ng/μl. Expression kinetics of mRNA transcripts in different seasons was studied using Brilliant SYBR Green QPCR technique and the data retrieved was analyzed by least-squares ANOVA. DNA sequencing by primer walking revealed four allelic variants of Hsp70 gene. Alignment study revealed one substitution in 5′UTR, six substitutions in coding region, and one addition in 3′UTR. The highest percent identity and negligible phylogenetic distance were found among the alleles and reference bovine sequences. The relative mRNA expression was significantly higher in summer when THI ≥ 84 than the spring and winter; fold change increased by 4.5 times in summer than the spring whereas found nearly half in winter. These findings can be useful for heat stress management in buffaloes and help in understanding the mechanism of thermo-regulation well.

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Acknowledgments

The authors are thankful to the Director, ICAR-Research Complex for Eastern Region, Patna, Bihar (India) for giving necessary permission towards the first author’s PhD dissertation research work to be carried out at its Division of Livestock and Fishery Management and duly acknowledged the kind cooperation sincerely extended by the Scientists and staff of this Division.

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Authors and Affiliations

  1. Department of Animal Genetics and Breeding, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Mohanpur, Nadia, West Bengal, 741252, India

    Birendra Kumar, Ajit Kumar Sahoo, Ananta Kumar Das & Subhash Taraphder

  2. Division of Livestock and Fishery Management, ICAR-Research Complex for Eastern Region, Patna, Bihar, 800014, India

    Shanker Dayal, Pradeep Kumar Ray & Rajni Kumari

  3. Department of Veterinary Biochemistry, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Mohanpur, Nadia, West Bengal, 741252, India

    Subhasis Batabyal

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  1. Birendra Kumar
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  2. Ajit Kumar Sahoo
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  3. Shanker Dayal
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  4. Ananta Kumar Das
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  8. Rajni Kumari
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Correspondence to Ananta Kumar Das.

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Kumar, B., Sahoo, A.K., Dayal, S. et al. Genetic profiling of Hsp70 gene in Murrah buffalo (Bubalus bubalis) under sub-tropical climate of India. Cell Stress and Chaperones 24, 1187–1195 (2019). https://doi.org/10.1007/s12192-019-01042-7

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  • DOI: https://doi.org/10.1007/s12192-019-01042-7

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