Abstract
Increased oxidative stress is a frequent feature in Duchenne muscular dystrophy (DMD). High reactive oxygen species (ROS) levels, associated with altered enzyme antioxidant activity, have been reported in dystrophic patients and mdx mice, an experimental model of DMD. In this study, we investigated the effects of coenzyme Q10 (CoQ10) on oxidative stress marker levels and calcium concentration in primary cultures of dystrophic muscle cells from mdx mice. Primary cultures of skeletal muscle cells from C57BL/10 and mdx mice were treated with coenzyme Q10 (5 μM) for 24 h. The untreated mdx and C57BL/10 muscle cells were used as controls. The MTT and live/dead cell assays showed that CoQ10 presented no cytotoxic effect on normal and dystrophic muscle cells. Intracellular calcium concentration, H2O2 production, 4-HNE, and SOD-2 levels were higher in mdx muscle cells. No significant difference in the catalase, GPx, and Gr levels was found between experimental groups. This study demonstrated that CoQ10 treatment was able to reduce levels of oxidative stress markers, such as H2O2, acting as an antioxidant, as well as decreasing abnormal intracellular calcium influx in dystrophic muscles cells. This study demonstrated that CoQ10 treatment was able to reduce levels of oxidative stress markers, such as H2O2, acting as an antioxidant, as well as decreasing abnormal intracellular calcium influx in dystrophic muscles cells. Our findings also suggest that the decrease of oxidative stress reduces the need for upregulation of antioxidant pathways, such as SOD and GSH.
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Funding
This study was financed in part by the Coordenação de Pessoal de Nivel Superior Brasil, (CAPES)–Finance Code 001, Fundação de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), CNPq and FAEPEX. L.H.R.M., A.R.F., and R.D.M. were the recipient of a FAPESP fellowship. D.S.M, T.A.H., and C.C.L are the recipient of a CAPES fellowship.
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Mizobuti, D.S., Fogaça, A.R., Moraes, F. et al. Coenzyme Q10 supplementation acts as antioxidant on dystrophic muscle cells. Cell Stress and Chaperones 24, 1175–1185 (2019). https://doi.org/10.1007/s12192-019-01039-2
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DOI: https://doi.org/10.1007/s12192-019-01039-2