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Coenzyme Q10 supplementation acts as antioxidant on dystrophic muscle cells

  • Daniela Sayuri Mizobuti
  • Aline Reis Fogaça
  • Fernanda dos Santos Rapucci Moraes
  • Luis Henrique Rapucci Moraes
  • Rafael Dias Mâncio
  • Túlio de Almeida Hermes
  • Aline Barbosa Macedo
  • Amanda Harduim Valduga
  • Caroline Caramano de Lourenço
  • Elaine Cristina Leite Pereira
  • Elaine MinatelEmail author
Original Paper
  • 90 Downloads

Abstract

Increased oxidative stress is a frequent feature in Duchenne muscular dystrophy (DMD). High reactive oxygen species (ROS) levels, associated with altered enzyme antioxidant activity, have been reported in dystrophic patients and mdx mice, an experimental model of DMD. In this study, we investigated the effects of coenzyme Q10 (CoQ10) on oxidative stress marker levels and calcium concentration in primary cultures of dystrophic muscle cells from mdx mice. Primary cultures of skeletal muscle cells from C57BL/10 and mdx mice were treated with coenzyme Q10 (5 μM) for 24 h. The untreated mdx and C57BL/10 muscle cells were used as controls. The MTT and live/dead cell assays showed that CoQ10 presented no cytotoxic effect on normal and dystrophic muscle cells. Intracellular calcium concentration, H2O2 production, 4-HNE, and SOD-2 levels were higher in mdx muscle cells. No significant difference in the catalase, GPx, and Gr levels was found between experimental groups. This study demonstrated that CoQ10 treatment was able to reduce levels of oxidative stress markers, such as H2O2, acting as an antioxidant, as well as decreasing abnormal intracellular calcium influx in dystrophic muscles cells. This study demonstrated that CoQ10 treatment was able to reduce levels of oxidative stress markers, such as H2O2, acting as an antioxidant, as well as decreasing abnormal intracellular calcium influx in dystrophic muscles cells. Our findings also suggest that the decrease of oxidative stress reduces the need for upregulation of antioxidant pathways, such as SOD and GSH.

Keywords

CoQ10 Oxidative stress mdx muscle cells Intracellular calcium Enzymatic antioxidant system 

Notes

Author contributions

The authors contributed substantially to conception and design, acquisition of data, analysis and interpretation of data. All authors participated in drafting the article, revised it critically for important intellectual content and gave final approval of the version to be submitted.

Funding information

This study was financed in part by the Coordenação de Pessoal de Nivel Superior Brasil, (CAPES)–Finance Code 001, Fundação de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), CNPq and FAEPEX. L.H.R.M., A.R.F., and R.D.M. were the recipient of a FAPESP fellowship. D.S.M, T.A.H., and C.C.L are the recipient of a CAPES fellowship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Cell Stress Society International 2019

Authors and Affiliations

  • Daniela Sayuri Mizobuti
    • 1
  • Aline Reis Fogaça
    • 1
  • Fernanda dos Santos Rapucci Moraes
    • 1
  • Luis Henrique Rapucci Moraes
    • 1
  • Rafael Dias Mâncio
    • 1
  • Túlio de Almeida Hermes
    • 1
  • Aline Barbosa Macedo
    • 1
  • Amanda Harduim Valduga
    • 1
  • Caroline Caramano de Lourenço
    • 1
  • Elaine Cristina Leite Pereira
    • 1
    • 2
  • Elaine Minatel
    • 1
    Email author
  1. 1.Departamento de Biologia Estrutural e FuncionalInstituto de Biologia, Universidade Estadual de Campinas (UNICAMP)CampinasBrazil
  2. 2.Faculdade de CeilandiaUniversidade de Brasília (UnB)BrasíliaBrazil

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