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Identification of an inositol-3-phosphate synthase 1-B gene (AccIPS1-B) from Apis cerana cerana and its role in abiotic stress

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Cell Stress and Chaperones Aims and scope

Abstract

Inositol phosphate synthase (IPS) is a rate-limiting enzyme in myo-inositol biosynthesis, which can regulate stress responses in plants and animals. However, there are few studies on the function of IPS in insects, especially in Apis cerana cerana. In this study, the inositol-3-phosphate synthase 1-B gene (AccIPS1-B) was isolated from Apis cerana cerana, and its connection to antioxidant defence was investigated. The open reading frame of AccIPS1-B was 1542 bp, encoding a 513 amino acid polypeptide. Quantitative real-time PCR analysis revealed that the expression level of AccIPS1-B was highest in pupae of Apis cerana cerana, and it was expressed at higher levels in the thorax than in other tissues tested. Moreover, the expression of AccIPS1-B was significantly upregulated by abiotic stresses. The recombinant AccIPS1-B also displayed significant tolerance to cumene hydroperoxide and HgCl2. In addition, knockdown of AccIPS1-B significantly suppressed the expression of most of the antioxidant genes and decreased the antioxidant enzymatic activities of SOD, POD, and GST. Taken together, these findings indicate that AccIPS1-B may be involved in the response to antioxidant defence and development in Apis cerana cerana.

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Funding

This work was financially supported by the Shandong Province Modern Agricultural Technology System Innovation Team Special Fund (SDAIT-24-04).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, 271018, Shandong, People’s Republic of China

    Yong Ni, Guilin Li, Xiaomin Ji, Yaqian Yang, Xingqi Guo & Qinghua Sun

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  1. Yong Ni
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  2. Guilin Li
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  3. Xiaomin Ji
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  4. Yaqian Yang
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  5. Xingqi Guo
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  6. Qinghua Sun
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Correspondence to Xingqi Guo or Qinghua Sun.

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Ni, Y., Li, G., Ji, X. et al. Identification of an inositol-3-phosphate synthase 1-B gene (AccIPS1-B) from Apis cerana cerana and its role in abiotic stress. Cell Stress and Chaperones 24, 1101–1113 (2019). https://doi.org/10.1007/s12192-019-01032-9

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