Testicular torsion and reperfusion: evidences for biochemical and molecular alterations
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This study was done in order to determine the molecular and biochemical alterations following testicular torsion (TT) and torsion-reperfusion (TR). For this purpose, 54 male Wistar rats were divided into five groups as control group (n = 6) and experimental group subjected to 1, 2, 4, and 8 h unilateral left torsion induction (n = 12 in each group). After induction of TT, testicular samples were collected from each group (n = 6), and the other six rats of each group underwent the same period of reperfusion after TT and then were sampled. Histological changes, the mRNA and protein expression of heat shock protein-70 (Hsp70), and caspase-3 were examined using reverse transcriptase-PCR (RT-PCR) and immunohistochemistry, respectively. Testicular total antioxidant capacity (TAC), glutathione peroxidase (GSH-px), and malondialdehyde (MDA) levels were evaluated. The mRNA damage and DNA fragmentation were assessed. The TT and TR significantly reduced differentiation and spermiogenesis indices (p < 0.05). The TT- and TR-induced groups exhibited a severe reduction in Hsp70 expression as well as remarkable enhancement in caspase-3 expression. The TAC and GSH-px levels were decreased and the MDA content was increased in TT- and TR-induced groups. Finally, the TT and TR enhanced mRNA damage and DNA fragmentation. The TT- and TR-induced damaging oxidative stress, diminished Hsp70 expression, and enhanced caspase-3 mRNA and protein levels result in apoptosis following 1, 2, and 4 h. Whereas, following 8 h, TT and TR initiate the necrosis by inducing energy depletion as well as severe mRNA damage.
KeywordsTorsion-reperfusion Hsp70 Caspase-3 Apoptosis Necrosis Rat
Authors wish to thank departments of Comparative Histology and Embryology and Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University.
- Dada R, Gupta N, Kucheria K (2002) Spermatogenic alterations in men with high testiculo epididymal temperatures. Indian J Human Genetics 8:20–25Google Scholar
- Ekici S, Dogan Ekici AI, Ozturk G, Benli Aksungar F, Sinanoglu O, Turan G, Luleci N (2012) Comparison of melatonin and ozone in the prevention of reperfusion injury following unilateral testicular torsion in rats. Urology 80:899–906. https://doi.org/10.1016/j.urology.2012.06.049 CrossRefPubMedGoogle Scholar
- Forlenza MJ, Miller GE (2006) Increased serum levels of 8-hydroxy-2′-deoxyguanosine in clinical depression. Psychosom Med 68:1–7. https://doi.org/10.1097/01.psy.0000195780.37277.2a CrossRefPubMedGoogle Scholar
- Gholirad S, Razi M, Hassani Bafrani H (2016) Tracing of zinc and iron in experimentally induced varicocele: correlation with oxidative, nitrosative and carbonyl stress. Andrologia 49(6). https://doi.org/10.1111/and.12687
- Gladden JD et al (2011) Novel insights into interactions between mitochondria and xanthine oxidase in acute cardiac volume overload. Free Radic Biol Med 51:1975–1984. https://doi.org/10.1016/j.freeradbiomed.2011.08.022 CrossRefPubMedPubMedCentralGoogle Scholar
- Khosravanian N, Razi M, Farokhi F, Khosravanian H (2014) Testosterone and vitamin E administration up-regulated varicocele-reduced Hsp70-2 protein expression and ameliorated biochemical alterations. J Assist Reprod Genet 31:341–354. https://doi.org/10.1007/s10815-013-0165-0 CrossRefPubMedPubMedCentralGoogle Scholar
- Lorenzini F, Tambara Filho R, Gomes RP, Martino-Andrade AJ, Erdmann TR, Matias JE (2012) Long-term effects of the testicular torsion on the spermatogenesis of the contralateral testis and the preventive value of the twisted testis orchiepididymectomy. Acta Cir Bras 27:388–395CrossRefPubMedGoogle Scholar
- Rezazadeh-Reyhani Z, Razi M, Malekinejad H, Sadrkhanlou R (2015) Cytotoxic effect of nanosilver particles on testicular tissue: evidence for biochemical stress and Hsp70-2 protein expression. Environ Toxicol Pharmacol 40:626–638. https://doi.org/10.1016/j.etap.2015.08.024 CrossRefPubMedGoogle Scholar