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Differential expression of microRNAs associated with thermal stress in Frieswal (Bos taurus x Bos indicus) crossbred dairy cattle

Cell Stress and Chaperones volume 23, pages 155–170 (2018)Cite this article

Abstract

Environmental temperature is one of the important abiotic factors that influence the normal physiological function and productive performance of dairy cattle. Temperature stress evokes complex responses that are essential for safeguarding of cellular integrity and animal health. Post-transcriptional regulation of gene expression by miRNA plays a key role cellular stress responses. The present study investigated the differential expression of miRNA in Frieswal (Holstein Friesian × Sahiwal) crossbred dairy cattle that are distinctly adapted to environmental temperature stress as they were evolved by using the temperate dairy breed Holstein Friesian. The results indicated that there was a significant variation in the physiological and biochemical indicators estimated under summer stress. The differential expression of miRNA was observed under heat stress when compared to the normal winter season. Out of the total 420 miRNAs, 65 were differentially expressed during peak summer temperatures. Most of these miRNAs were found to target heat shock responsive genes especially members of heat shock protein (HSP) family, and network analysis revealed most of them having stress-mediated effects on signaling mechanisms. Being greater in their expression profile during peak summer, bta-miR-2898 was chosen for reporter assay to identify its effect on the target HSPB8 (heat shock protein 22) gene in stressed bovine PBMC cell cultured model. Comprehensive understanding of the biological regulation of stress responsive mechanism is critical for developing approaches to reduce the production losses due to environmental heat stress in dairy cattle.

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Acknowledgements

The authors are thankful to the Director, ICAR-CIRC, Meerut for providing necessary facilities to carry out the present research. Authors acknowledge the Science and Engineering Research Board, Government of India, for providing financial support under the project YSS/2014/000279 to RD. We are also thankful to Military Farm, Meerut, India, for providing experimental animals; Ome Research Laboratory, Anand Agricultural University, Gujrat, India for deep sequencing.

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Author notes

  1. Gyanendra Singh Sengar and Rajib Deb contributed equally

Authors and Affiliations

  1. Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250 001, India

    Gyanendra Singh Sengar, Rajib Deb, Umesh Singh, T. V. Raja, Rani Alex, R. R. Alyethodi, Ashish Kumar, Sushil Kumar & Rani Singh

  2. Sam Higginbottom University of Agriculture Technology and Science, Allahabad, India

    Gyanendra Singh Sengar & Rajiv Kant

  3. School of Atmospheric Stress Management, National Institute of Abiotic Stress Management, Baramati, Maharashtra, India

    Basavraj Sajjanar

  4. Department of Animal Biotechnology, Anand Agricultural University, Anand, Gujarat, India

    Subhash J. Jakhesara & C. G. Joshi

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  1. Gyanendra Singh Sengar
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Correspondence to Gyanendra Singh Sengar or Rajib Deb.

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All the experimental procedures involving animals were approved by the Institutional Animal Ethics Committee (IAEC)

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Sengar, G.S., Deb, R., Singh, U. et al. Differential expression of microRNAs associated with thermal stress in Frieswal (Bos taurus x Bos indicus) crossbred dairy cattle. Cell Stress and Chaperones 23, 155–170 (2018). https://doi.org/10.1007/s12192-017-0833-6

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  • DOI: https://doi.org/10.1007/s12192-017-0833-6

Keywords

  • Heat stress
  • miRNAs
  • Frieswal
  • bta-miR-2898
  • HSPB8