Abstract
As the population ages, there is a critical need to uncover strategies to combat diseases of aging. Studies in the soil-dwelling nematode Caenorhabditis elegans have demonstrated the protective effects of coffee extract and caffeine in promoting the induction of conserved longevity pathways including the insulin-like signaling pathway and the oxidative stress response. We were interested in determining the effects of coffee and caffeine treatment on the regulation of the heat shock response. The heat shock response is a highly conserved cellular response that functions as a cytoprotective mechanism during stress, mediated by the heat shock transcription factor HSF-1. In the worm, HSF-1 not only promotes protection against stress but is also essential for development and longevity. Induction of the heat shock response has been suggested to be beneficial for diseases of protein conformation by preventing protein misfolding and aggregation, and as such has been proposed as a therapeutic target for age-associated neurodegenerative disorders. In this study, we demonstrate that coffee is a potent, dose-dependent, inducer of the heat shock response. Treatment with a moderate dose of pure caffeine was also able to induce the heat shock response, indicating caffeine as an important component within coffee for producing this response. The effects that we observe with both coffee and pure caffeine on the heat shock response are both dependent on HSF-1. In a C. elegans Huntington’s disease model, worms treated with caffeine were protected from polyglutamine aggregates and toxicity, an effect that was also HSF-1-dependent. In conclusion, these results demonstrate caffeinated coffee, and pure caffeine, as protective substances that promote proteostasis through induction of the heat shock response.
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Ascherio A, Zhang SM, Hernan MA, Kawachi I, Colditz GA, Speizer FE, Willett WC (2001) Prospective study of caffeine consumption and risk of Parkinson’s disease in men and women. Ann Neurol 50:56–63
Ashrafi K, Chang FY, Watts JL, Fraser AG, Kamath RS, Ahringer J, Ruvkun G (2003) Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes. Nature 421:268–272. doi:10.1038/nature01279
Balch WE, Morimoto RI, Dillin A, Kelly JW (2008) Adapting proteostasis for disease intervention. Science 319:916–919. doi:10.1126/science.1141448
Barna J, Princz A, Kosztelnik M, Hargitai B, Takacs-Vellai K, Vellai T (2012) Heat shock factor-1 intertwines insulin/IGF-1, TGF-beta and cGMP signaling to control development and aging. BMC developmental biology 12:32. doi:10.1186/1471-213X-12-32
Bookout AL, Mangelsdorf DJ (2003) Quantitative real-time PCR protocol for analysis of nuclear receptor signaling pathways. Nucl Recept Signal 1:e012. doi:10.1621/nrs.01012
Bridi JC, Barros AG, Sampaio LR, Ferreira JC, Antunes Soares FA, Romano-Silva MA (2015) Lifespan extension induced by caffeine in Caenorhabditis elegans is partially dependent on adenosine signaling. Front Aging Neurosci 7:220. doi:10.3389/fnagi.2015.00220
Brunquell J, Bowers P, Westerheide SD (2014) Fluorodeoxyuridine enhances the heat shock response and decreases polyglutamine aggregation in an HSF-1-dependent manner in Caenorhabditis elegans. Mech Ageing Dev 141-142:1–4. doi:10.1016/j.mad.2014.08.002
Calamini B, Morimoto RI (2012) Protein homeostasis as a therapeutic target for diseases of protein conformation. Curr Top Med Chem 12:2623–2640
Chen X, Barclay JW, Burgoyne RD, Morgan A (2015) Using C. elegans to discover therapeutic compounds for ageing-associated neurodegenerative diseases. Chem Cent J 9:65. doi:10.1186/s13065-015-0143-y
Chiang WC, Ching TT, Lee HC, Mousigian C, Hsu AL (2012) HSF-1 regulators DDL-1/2 link insulin-like signaling to heat-shock responses and modulation of longevity. Cell 148:322–334. doi:10.1016/j.cell.2011.12.019
Coburn C, Gems D (2013) The mysterious case of the C. elegans gut granule: death fluorescence, anthranilic acid and the kynurenine pathway. Front Genet 4:151. doi:10.3389/fgene.2013.00151
Cunha RA, Agostinho PM (2010) Chronic caffeine consumption prevents memory disturbance in different animal models of memory decline. J Alzheimers Dis : JAD 20(Suppl 1):S95–116. doi:10.3233/JAD-2010-1408
Dayalan Naidu S, Dinkova-Kostova AT (2017) Regulation of the mammalian heat shock factor 1. FEBS J. doi:10.1111/febs.13999
Dostal V, Roberts CM, Link CD (2010) Genetic mechanisms of coffee extract protection in a Caenorhabditis elegans model of beta-amyloid peptide toxicity. Genetics 186:857–866. doi:10.1534/genetics.110.120436
Elmenhorst D, Meyer PT, Matusch A, Winz OH, Bauer A (2012) Caffeine occupancy of human cerebral A1 adenosine receptors: in vivo quantification with 18F-CPFPX and PET. J Nucl Med : Off Publication Soc Nucl Med 53:1723–1729. doi:10.2967/jnumed.112.105114
Eskelinen MH, Kivipelto M (2010) Caffeine as a protective factor in dementia and Alzheimer’s disease. J Alzheimers Dis : JAD 20(Suppl 1):S167–S174. doi:10.3233/JAD-2010-1404
Farah A, Donangelo CM (2006) Phenolic compounds in coffee Brazilian. J Plant Physiol 18:23–36
Fredholm BB, Battig K, Holmen J, Nehlig A, Zvartau EE (1999) Actions of caffeine in the brain with special reference to factors that contribute to its widespread use Pharmacol Rev 51:83–133
Frydman J (2001) Folding of newly translated proteins in vivo: the role of molecular chaperones. Annu Rev Biochem 70:603–647. doi:10.1146/annurev.biochem.70.1.603
Garigan D, Hsu AL, Fraser AG, Kamath RS, Ahringer J, Kenyon C (2002) Genetic analysis of tissue aging in Caenorhabditis elegans: a role for heat-shock factor and bacterial proliferation. Genetics 161:1101–1112
Gidalevitz T, Prahlad V, Morimoto RI (2011) The stress of protein misfolding: from single cells to multicellular organisms. Cold Spring Harb Perspect Biol 3 doi:10.1101/cshperspect.a009704
Hajdu-Cronin YM, Chen WJ, Sternberg PW (2004) The L-type cyclin CYL-1 and the heat-shock-factor HSF-1 are required for heat-shock-induced protein expression in Caenorhabditis elegans. Genetics 168:1937–1949. doi:10.1534/genetics.104.028423
Hameleers PA, Van Boxtel MP, Hogervorst E, Riedel WJ, Houx PJ, Buntinx F, Jolles J (2000) Habitual caffeine consumption and its relation to memory, attention, planning capacity and psychomotor performance across multiple age groups. Hum Psychopharmacol 15:573–581. doi:10.1002/hup.218
Hartl FU, Bracher A, Hayer-Hartl M (2011) Molecular chaperones in protein folding and proteostasis. Nature 475:324–332. doi:10.1038/nature10317
Houessou JK, Benac C, Delteil C, Camel V (2005) Determination of polycyclic aromatic hydrocarbons in coffee brew using solid-phase extraction. J Agric Food Chem 53:871–879. doi:10.1021/jf048633a
Hsu AL, Murphy CT, Kenyon C (2003) Regulation of aging and age-related disease by DAF-16 and heat-shock factor. Science 300:1142–1145. doi:10.1126/science.1083701
Jurivich DA, Sistonen L, Sarge KD, Morimoto RI (1994) Arachidonate is a potent modulator of human heat shock gene transcription. Proc Natl Acad Sci U S A 91:2280–2284
Kamath RS et al (2003) Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 421:231–237. doi:10.1038/nature01278
Lee BS, Chen J, Angelidis C, Jurivich DA, Morimoto RI (1995) Pharmacological modulation of heat shock factor 1 by antiinflammatory drugs results in protection against stress-induced cellular damage. Proc Natl Acad Sci U S A 92:7207–7211
Lublin A et al (2011) FDA-approved drugs that protect mammalian neurons from glucose toxicity slow aging dependent on cbp and protect against proteotoxicity. PloS one 6:e27762. doi:10.1371/journal.pone.0027762
McCall AL, Millington WR, Wurtman RJ (1982) Blood-brain barrier transport of caffeine: dose-related restriction of adenine transport. Life Sciences 31:2709–2715
Moeenfard M, Erny GL, Alves A (2016) Variability of some diterpene esters in coffee beverages as influenced by brewing procedures. J Food Sci Technol 53:3916–3927. doi:10.1007/s13197-016-2378-6
Morley JF, Brignull HR, Weyers JJ, Morimoto RI (2002) The threshold for polyglutamine-expansion protein aggregation and cellular toxicity is dynamic and influenced by aging in Caenorhabditis elegans. Proc Natl Acad Sci U S A 99:10417–10422. doi:10.1073/pnas.152161099
Morley JF, Morimoto RI (2004) Regulation of longevity in Caenorhabditis elegans by heat shock factor and molecular chaperones. Mol Biol Cell 15:657–664. doi:10.1091/mbc.E03-07-0532
Morton EA, Lamitina T (2013) Caenorhabditis elegans HSF-1 is an essential nuclear protein that forms stress granule-like structures following heat shock. Aging Cell 12:112–120. doi:10.1111/acel.12024
Muchowski PJ (2002) Protein misfolding, amyloid formation, and neurodegeneration: a critical role for molecular chaperones? Neuron 35:9–12
Muchowski PJ, Wacker JL (2005) Modulation of neurodegeneration by molecular chaperones. Nat Rev Neurosci 6:11–22. doi:10.1038/nrn1587
Neef DW, Turski ML, Thiele DJ (2010) Modulation of heat shock transcription factor 1 as a therapeutic target for small molecule intervention in neurodegenerative disease. PLoS Biol 8:e1000291. doi:10.1371/journal.pbio.1000291
Nehlig A, Daval JL, Debry G (1992) Caffeine and the central nervous system: mechanisms of action, biochemical, metabolic and psychostimulant effects. Brain Res Brain Res Rev 17:139–170
Oba S, Nagata C, Nakamura K, Fujii K, Kawachi T, Takatsuka N, Shimizu H (2010) Consumption of coffee, green tea, oolong tea, black tea, chocolate snacks and the caffeine content in relation to risk of diabetes in Japanese men and women. Br J Nutr 103:453–459. doi:10.1017/S0007114509991966
Paganini-Hill A, Kawas CH, Corrada MM (2007) Non-alcoholic beverage and caffeine consumption and mortality: the Leisure World Cohort Study. Prev Med 44:305–310. doi:10.1016/j.ypmed.2006.12.011
Prahlad V, Cornelius T, Morimoto RI (2008) Regulation of the cellular heat shock response in Caenorhabditis elegans by thermosensory neurons. Science 320:811–814. doi:10.1126/science.1156093
Raynes R, Leckey BD Jr, Nguyen K, Westerheide SD (2012) Heat shock and caloric restriction have a synergistic effect on the heat shock response in a sir2.1-dependent manner in Caenorhabditis elegans. J Biol Chem 287:29045–29053. doi:10.1074/jbc.M112.353714
Roh HC, Collier S, Guthrie J, Robertson JD, Kornfeld K (2012) Lysosome-related organelles in intestinal cells are a zinc storage site in C. elegans. Cell metabolism 15:88–99. doi:10.1016/j.cmet.2011.12.003
Santos C, Lunet N, Azevedo A, de Mendonca A, Ritchie K, Barros H (2010) Caffeine intake is associated with a lower risk of cognitive decline: a cohort study from Portugal. J Alzheimers Dis : JAD 20(Suppl 1):S175–S185. doi:10.3233/JAD-2010-091303
Satyal SH, Schmidt E, Kitagawa K, Sondheimer N, Lindquist S, Kramer JM, Morimoto RI (2000) Polyglutamine aggregates alter protein folding homeostasis in Caenorhabditis elegans Proc Natl Acad Sci U S A 97:5750–5755
Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9:671–675
Schroeder LK et al (2007) Function of the Caenorhabditis elegans ABC transporter PGP-2 in the biogenesis of a lysosome-related fat storage organelle. Mol Biol Cell 18:995–1008. doi:10.1091/mbc.E06-08-0685
Shi D, Nikodijevic O, Jacobson KA, Daly JW (1993) Chronic caffeine alters the density of adenosine, adrenergic, cholinergic, GABA, and serotonin receptors and calcium channels in mouse brain. Cell Mol Neurobiol 13:247–261
Silva MC, Fox S, Beam M, Thakkar H, Amaral MD, Morimoto RI (2011) A genetic screening strategy identifies novel regulators of the proteostasis network. PLoS Genet 7:e1002438. doi:10.1371/journal.pgen.1002438
Singh V, Aballay A (2006) Heat-shock transcription factor (HSF)-1 pathway required for Caenorhabditis elegans immunity. Proc Natl Acad Sci U S A 103:13092–13097. doi:10.1073/pnas.0604050103
Sutphin GL, Bishop E, Yanos ME, Moller RM, Kaeberlein M (2012) Caffeine extends life span, improves healthspan, and delays age-associated pathology in Caenorhabditis elegans. Longevity Healthspan 1:9. doi:10.1186/2046-2395-1-9
Tatum MC et al (2015) Neuronal serotonin release triggers the heat shock response in C. elegans in the absence of temperature increase. Curr Biol 25:163–174. doi:10.1016/j.cub.2014.11.040
Torok Z et al (2003) Heat shock protein coinducers with no effect on protein denaturation specifically modulate the membrane lipid phase. Proc Natl Acad Sci U S A 100:3131–3136
Trinh K, Andrews L, Krause J, Hanak T, Lee D, Gelb M, Pallanck L (2010) Decaffeinated coffee and nicotine-free tobacco provide neuroprotection in Drosophila models of Parkinson's disease through an NRF2-dependent mechanism. J Neurosci : Off J Soc Neurosci 30:5525–5532. doi:10.1523/JNEUROSCI.4777-09.2010
van Oosten-Hawle P, Morimoto RI (2014) Transcellular chaperone signaling: an organismal strategy for integrated cell stress responses. J Exp Biol 217:129–136. doi:10.1242/jeb.091249
van Oosten-Hawle P, Porter RS, Morimoto RI (2013) Regulation of organismal proteostasis by transcellular chaperone signaling. Cell 153:1366–1378. doi:10.1016/j.cell.2013.05.015
West JD, Wang Y, Morano KA (2012) Small molecule activators of the heat shock response: chemical properties, molecular targets, and therapeutic promise. Chem Res Toxicol 25:2036–2053. doi:10.1021/tx300264x
Westerheide SD et al (2004) Celastrols as inducers of the heat shock response and cytoprotection. J Biol Chem 279:56053–56060
Westerheide SD, Morimoto RI (2005) Heat shock response modulators as therapeutic tools for diseases of protein conformation. J Biol Chem 280:33097–33100. doi:10.1074/jbc.R500010200
Zack GW, Rogers WE, Latt SA (1977) Automatic measurement of sister chromatid exchange frequency. J Histochem Cytochem : Off J Histochem Soc 25:741–753
Acknowledgements
We would like to thank Dr. R. I. Morimoto (Northwestern University) for providing the pC12C8.1::GFP and Q35::YFP C. elegans strains, and the Caenorhabditis Genetics Center, which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440), for providing the N2 strain. We would also like to thank Dr. R. Raynes (Amgen) for helpful discussion.
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This work was supported by a departmental start-up grant from the Department of Cell Biology, Microbiology, and Molecular Biology at the University of South Florida to Sandy D. Westerheide.
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Brunquell, J., Morris, S., Snyder, A. et al. Coffee extract and caffeine enhance the heat shock response and promote proteostasis in an HSF-1-dependent manner in Caenorhabditis elegans . Cell Stress and Chaperones 23, 65–75 (2018). https://doi.org/10.1007/s12192-017-0824-7
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DOI: https://doi.org/10.1007/s12192-017-0824-7