Cell Stress and Chaperones

, Volume 22, Issue 4, pp 653–663 | Cite as

Upregulation and phosphorylation of HspB1/Hsp25 and HspB5/αB-crystallin after transient middle cerebral artery occlusion in rats

  • Britta Bartelt-Kirbach
  • Alexander Slowik
  • Cordian Beyer
  • Nikola Golenhofen


Ischemic stroke leads to cellular dysfunction, cell death, and devastating clinical outcomes. The cells of the brain react to such a cellular stress by a stress response with an upregulation of heat shock proteins resulting in activation of endogenous neuroprotective capacities. Several members of the family of small heat shock proteins (HspBs) have been shown to be neuroprotective. However, yet no systematic study examined all HspBs during cerebral ischemia. Here, we performed a comprehensive comparative study comprising all HspBs in an animal model of stroke, i.e., 1 h transient middle cerebral artery occlusion followed by 23 h of reperfusion. On the mRNA level out of the 11 HspBs investigated, HspB1/Hsp25, HspB3, HspB4/αA-crystallin, HspB5/αB-crystallin, HspB7/cvHsp, and HspB8/Hsp22 were significantly upregulated in the peri-infarct region of the cerebral cortex of infarcted hemispheres. HspB1 and HspB5 reached the highest mRNA levels and were also upregulated at the protein level, suggesting that these HspBs might be functionally most relevant. Interestingly, in the infarcted cortex, both HspB1 and HspB5 were mainly allocated to neurons and to a lesser extent to glial cells. Additionally, both proteins were found to be phosphorylated in response to ischemia. Our data suggest that among all HspBs, HspB1 and HspB5 might be most important in the neuronal stress response to ischemia/reperfusion injury in the brain and might be involved in neuroprotection.


Stroke Ischemia Neuronal stress response Heat shock proteins HspB 



We thank Bianca Mekle, Diana Reinhard, and Stephanie Sues for their excellent technical assistance.

Compliance with ethical standards

Animal care and experimental procedures were formally approved by the Review Board for the Care of Animal Subjects of the district government (North Rhine-Westphalia, Germany) and bred and maintained in a pathogen-free environment in a 12-h day and 12-h night cycle prior experiments.

Supplementary material

12192_2017_794_MOESM1_ESM.pdf (134 kb)
Electronic resource 1 (PDF 133 kb)
12192_2017_794_MOESM2_ESM.pdf (77 kb)
Electronic resource 2 (PDF 77 kb)
12192_2017_794_MOESM3_ESM.pdf (139 kb)
Electronic resource 3 (PDF 139 kb)


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Copyright information

© Cell Stress Society International 2017

Authors and Affiliations

  1. 1.Institute of Anatomy and Cell BiologyUniversity of UlmUlmGermany
  2. 2.Institute of NeuroanatomyRWTH Aachen UniversityAachenGermany

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