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High-dose green tea polyphenols induce nephrotoxicity in dextran sulfate sodium-induced colitis mice by down-regulation of antioxidant enzymes and heat-shock protein expressions

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Cell Stress and Chaperones Aims and scope

Abstract

Previously, we reported that oral feeding of 1% green tea polyphenols (GTPs) aggravated the dextran sulfate sodium (DSS)-induced colitis in mice. In the present study, we assessed the toxicity of 1% GTPs in several organs from normal and DSS-exposed mice. Sixty-two male ICR mice were initially divided into four groups. Non-treated group (group 1, n = 15) was given standard diet and water, GTPs (group 2, n = 15) received 1% GTPs in diet and water, DSS (group 3, n = 15) received diet and 5% DSS in water, and GTPs + DSS group (group 4, n = 17) received 1% GTPs in diet and 5% DSS in water. We found that group 4 significantly increased (P < 0.05) kidney weight, the levels of serum creatinine and thiobarbituric acid-reactive substances in both kidney and liver, as compared with those in group 3. The mRNA expression levels of antioxidant enzymes and heat-shock proteins (HSPs) in group 4 were lower than those of group 3. For instance, heme oxygenase-1 (HO-1), HSP27, and 90 mRNA in the kidney of group 4 were dramatically down-regulated as compared with those of group 3. Furthermore, 1% GTPs diet decreased the expression of HO-1, NAD(P)H:quinone oxidoreductase 1 (NQO1) and HSP90 in kidney and liver of non-treated mice. Taken together, our results indicate that high-dose GTPs diet disrupts kidney functions through the reduction of antioxidant enzymes and heat-shock protein expressions in not only colitis but also non-treated ICR mice.

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Acknowledgements

This work was supported by the Programme for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry and by Research and Development Projects for Application in Promoting New Policy of Agriculture Forestry and Fisheries, Ministry of Agriculture, Forestry and Fisheries, Japan (Grant No. 23005).

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Authors and Affiliations

  1. Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan

    Hirofumi Inoue, Satoko Akiyama & Akira Murakami

  2. National Institute of Vegetable and Tea Sciences, National Agriculture and Food Research Organization, 2769 Kanaya, Shimada, Shizuoka, 428-8501, Japan

    Mari Maeda-Yamamoto & Atsushi Nesumi

  3. The Tohkai Cytopathology Institute: Cancer Research and Prevention (TCI-CaPP), 4-33 Minami-Uzura, Gifu, 500-8285, Japan

    Takuji Tanaka

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  1. Hirofumi Inoue
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  2. Satoko Akiyama
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  4. Atsushi Nesumi
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  5. Takuji Tanaka
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Correspondence to Akira Murakami.

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Inoue, H., Akiyama, S., Maeda-Yamamoto, M. et al. High-dose green tea polyphenols induce nephrotoxicity in dextran sulfate sodium-induced colitis mice by down-regulation of antioxidant enzymes and heat-shock protein expressions. Cell Stress and Chaperones 16, 653–662 (2011). https://doi.org/10.1007/s12192-011-0280-8

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