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Elevated serum levels of heat shock protein 70 can be detected after radiofrequency ablation

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Cell Stress and Chaperones Aims and scope

Abstract

Due to their adjuvant effect and their ability to chaperone tumor-associated peptides, heat shock proteins constitute a potent alarm signal for the immune system and can lead to activation of anti-tumor T-cell immunity. Radiofrequency ablation has been reported to induce heat shock protein expression especially that of heat shock protein 70 in sublethally damaged tumor cells. In this study, we evaluated the release of heat shock protein 70 into the serum of cancer-bearing patients directly after radiofrequency ablation. Sera of 22 patients undergoing radiofrequency ablation for the treatment of primary and secondary malignancies of the liver, kidney, and lung, as well as control sera of 20 patients undergoing diagnostic liver biopsy were analyzed using a manufactured heat shock protein 70 ELISA. A significant increase in serum levels of heat shock protein 70 was detectable in the patient cohort 1 day after radiofrequency ablation. More than a twofold increase was observed in nine out of 22 patients, which tended to correlate with favorable clinical outcome. No patient of the control group revealed a comparable increase. Radiofrequency ablation can lead to a release of heat shock protein 70 into the serum, which is transiently detectable 1 day after treatment. Elevated heat shock protein 70 serum levels may constitute a biomarker for favorable clinical outcome.

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Acknowledgments

We wish to thank Lynne Yakes for editorial assistance as well as Hansjoerg Schild for helpful discussion.

Author information

Authors and Affiliations

  1. Interfakultaeres Institut fuer Zellbiologie, Abteilung Immunologie, Auf der Morgenstelle 15, 72076, Tuebingen, Germany

    Sebastian P. Haen, Cécile Gouttefangeas & Hans-Georg Rammensee

  2. Innere Medizin II, Onkologie, Haematologie, Immunologie, Rheumatologie und Pulmologie, Medizinische Universitaetsklinik Tuebingen, Otfried Mueller Str. 10, 72076, Tuebingen, Germany

    Sebastian P. Haen

  3. Abteilung fuer Diagnostische und Interventionelle Radiologie, Radiologische Universitaetsklinik Tuebingen, Hoppe-Seyler-Str. 3, 72076, Tuebingen, Germany

    Diethard Schmidt, Andreas Boss, Stephan Clasen & Philippe L. Pereira

  4. Innere Medizin I, Gastroenterologie, Hepatologie und Infektiologie, Medizinische Universitaetsklinik Tuebingen, Otfried Mueller Str. 10, 72076, Tuebingen, Germany

    Alexandra von Herbay

  5. Klinik fuer Thorax, Herz- und Gefaesschirurgie, Universitaetsklinikum Tuebingen, Hoppe-Seyler-Str. 3, 72076, Tuebingen, Germany

    Bora Kosan & Hermann Aebert

  6. Herzogin Elisabeth Krankenhaus, Leipziger Str. 24, 38124, Braunschweig, Germany

    Alexandra von Herbay

  7. Klinik Schillerhoehe, Thoraxchirurgie, Solitudestr. 12, 70839, Gerlingen, Germany

    Bora Kosan

  8. Schwarzwald Baar Klinikum, Kliniken Donaueschingen, Sonnhaldenstr. 2, 78166, Donaueschingen, Germany

    Hermann Aebert

  9. SLK Kliniken Heilbronn, Klinik fuer Radiologie, Minimalinvasive Therapien und Nuklearmedizin, Am Gesundbrunnen 20–26, 74078, Heilbronn, Germany

    Philippe L. Pereira

Authors
  1. Sebastian P. Haen
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  2. Cécile Gouttefangeas
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  3. Diethard Schmidt
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  4. Andreas Boss
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  5. Stephan Clasen
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  6. Alexandra von Herbay
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  7. Bora Kosan
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  8. Hermann Aebert
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  9. Philippe L. Pereira
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  10. Hans-Georg Rammensee
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Corresponding author

Correspondence to Sebastian P. Haen.

Additional information

Sebastian P. Haen and Cécile Gouttefangeas contributed equally to this work.

This work was supported by a grant from the University of Tuebingen, Fortüne project No. 1530-0-0 and from third-party funding donated by Hölle and Hüttner AG.

Sebastian P. Haen is supported by the Deutsche José Carreras Leukemia Foundation.

No authors have any commercial interest in this report.

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Haen, S.P., Gouttefangeas, C., Schmidt, D. et al. Elevated serum levels of heat shock protein 70 can be detected after radiofrequency ablation. Cell Stress and Chaperones 16, 495–504 (2011). https://doi.org/10.1007/s12192-011-0261-y

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