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The molecular characterization and expression of heat shock protein 90 (Hsp90) and 26 (Hsp26) cDNAs in sea cucumber (Apostichopus japonicus)

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Cell Stress and Chaperones Aims and scope

Abstract

The heat shock proteins (HSPs) are a family of proteins whose expression is enhanced in response to environmental stressors. The Apostichopus japonicus hsp90 and hsp26 genes were cloned using expressed sequence tag and rapid amplification of cDNA ends techniques. The full-length cDNA of Aphsp90 and Aphsp26 contains 3,458 and 1,688 nucleotides encoding 720 and 236 amino acids, respectively. Multiple alignments indicated that the deduced amino acid sequences of ApHsp90 and ApHsp26 shared a high level of identity with Hsp90 and small SHPs (sHSPs) sequences of zebrafish, ant, acorn worms, etc., and shared identical structural features with Hsp90 and sHSPs. The expression profiles of these two genes under heat treatment were investigated by real-time quantitative PCR. It was found that the messenger RNA (mRNA) transcripts of the two A. japonicus genes varied among different tissues under normal conditions and heat shock, and that the mRNA expression of the two genes was higher in the intestine compared to other tissues. Heat shock significantly elevated the expression of Aphsp90 and Aphsp26 mRNA in a temperature- and time-dependent manner. The results indicate that Aphsp90 and Aphsp26 played important roles in mediating the environmental stress in A. japonicus.

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Acknowledgments

This work was supported by the agricultural seed project of Shan Dong province and the National Key Technology R&D Program (2006AA10A411).

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Authors and Affiliations

  1. Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road 7, Qingdao, Shandong, 266071, China

    Huan Zhao, Hongsheng Yang, Heling Zhao & Tianming Wang

  2. Graduate University, Chinese Academy of Sciences, Beijing, 100049, China

    Huan Zhao, Heling Zhao & Tianming Wang

  3. Department of Developmental and Cell Biology, University of California, Irvine, CA, CA92697, USA

    Muyan Chen

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  1. Huan Zhao
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  2. Hongsheng Yang
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  3. Heling Zhao
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  5. Tianming Wang
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Correspondence to Hongsheng Yang.

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Zhao, H., Yang, H., Zhao, H. et al. The molecular characterization and expression of heat shock protein 90 (Hsp90) and 26 (Hsp26) cDNAs in sea cucumber (Apostichopus japonicus). Cell Stress and Chaperones 16, 481–493 (2011). https://doi.org/10.1007/s12192-011-0260-z

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