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Cell Stress and Chaperones

, Volume 15, Issue 6, pp 877–884 | Cite as

Hsp60 and Hsp10 increase in colon mucosa of Crohn’s disease and ulcerative colitis

  • Vito Rodolico
  • Giovanni Tomasello
  • Monica Zerilli
  • Anna Martorana
  • Alessandro Pitruzzella
  • Antonella Marino Gammazza
  • Sabrina David
  • Giovanni Zummo
  • Provvidenza Damiani
  • Salvatore Accomando
  • Everly Conway de Macario
  • Alberto J. L. Macario
  • Francesco CappelloEmail author
Original Paper

Abstract

The purpose of this work was to determine in colon mucosa of Crohn’s disease (CD) and ulcerative colitis (UC) in relapse: a) the levels of the chaperonins Hsp60 and Hsp10; b) the quantity of inflammatory cells; and c) if the levels of chaperonins parallel those of inflammation cells. Twenty cases of CD and UC and twenty normal controls (NC) were studied using immunohistochemistry, Western blotting and immunofluorescence. Immunohistochemically, Hsp60 and Hsp10 were increased in both inflammatory bowel diseases (IBD) compared to NC. These results were confirmed by Western blotting. Hsp60 and Hsp10 occurred in the cytoplasm of epithelial cells in CD and UC but not in NC. Hsp60 and Hsp10 co-localised to epithelial cells of mucosal glands but not always in connective tissue cells of lamina propria, where only Hsp60 or, less often, Hsp10 was found. Cells typical of inflammation were significantly more abundant in CD and UC than in NC. Since chaperonins are key factors in the activation of the immune system leading to inflammation, we propose that they play a central role in the pathogenesis of the two diseases, which, consequently, ought to be studied as chaperonopathies.

Keywords

Chaperonins Inflammation IBD Chaperonopathies Autoimmunity 

Notes

Acknowledgements

This work was supported by funds of University of Palermo (VR, SD, GZ and FC) and Istituto Euro-Mediterraneo di Scienza e Tecnologia (FC).

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Copyright information

© Cell Stress Society International 2010

Authors and Affiliations

  • Vito Rodolico
    • 1
  • Giovanni Tomasello
    • 2
  • Monica Zerilli
    • 1
  • Anna Martorana
    • 1
  • Alessandro Pitruzzella
    • 3
  • Antonella Marino Gammazza
    • 3
  • Sabrina David
    • 3
  • Giovanni Zummo
    • 3
  • Provvidenza Damiani
    • 4
  • Salvatore Accomando
    • 5
  • Everly Conway de Macario
    • 6
  • Alberto J. L. Macario
    • 6
  • Francesco Cappello
    • 3
    • 7
    Email author
  1. 1.Dipartimento di Patologia UmanaUniversità degli Studi di PalermoPalermoItaly
  2. 2.Dipartimento di Chirurgia Generale, d’Urgenza e Trapianti d’OrganoUniversità degli Studi di PalermoPalermoItaly
  3. 3.Dipartimento di Biomedicina Sperimentale e Neuroscienze ClinicheUniversità degli Studi di PalermoPalermoItaly
  4. 4.Dipartimento di Medicina Interna, Malattie Cardiovascolari e NefrourologicheUniversità degli Studi di PalermoPalermoItaly
  5. 5.Dipartimento Materno-InfantileUniversità degli Studi di PalermoPalermoItaly
  6. 6.University of Maryland, IMETBaltimoreUSA
  7. 7.Istituto Euro-Mediterraneo di Scienza e Tecnologia (IEMEST)PalermoItaly

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