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Expression of uncoupling protein 3 in mitochondria protects against stress-induced myocardial injury: a proteomic study

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Cell Stress and Chaperones Aims and scope

Abstract

It has been confirmed that stress plays an important role in the induction and development of cardiovascular diseases, but its mechanism and molecular basis remain unknown. In the present study, a myocardial injury model induced by restraint stress was established in rat. To screen for the related proteins involved in stress-induced myocardial injury, proteomic techniques based on 2-DE and mass spectrometry were used. In our results, ten proteins were found to be altered. The expression of eight of these proteins was increased after restraint stress, including cardiac myosin heavy chain, dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial aldehyde dehydrogenase, H+-transporting ATP synthase, albumin, and apolipoprotein A-I precursor. The expression of uncoupling protein 3 (UCP3) and mitochondrial aconitase was decreased. Most of the proteins were related to energy metabolism. Further research indicated that UCP3 may mediate the myocardial cell response induced by restraint stress.

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Abbreviations

ROS:

reactive oxygen species

NE:

norepinephrine

MEM:

minimum essential medium

PTP:

permeability transition pore

2-DE:

two-dimensional gel electrophoresis

UCP3:

uncoupling protein 3

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Acknowledgment

This work was supported in part by grants from the National Natural Science Foundation of China (No. 30770843, No. 30430590).

Conflict of interest

None declared.

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Authors and Affiliations

  1. Key Laboratory of Stress Medicine, Tianjing Institute of Hygiene and Environmental Medicine, 1 Dali Road, Heping District, Tianjing, 300050, China

    Xinxing Wang, Jingbo Gong, Xiaohua Liu, Rui Zhan, Ruirui Kong, Yun Zhao, Di Wan, Xue Leng, Ming Chen & Lingjia Qian

  2. Department of Stress Medicine, Institute of Health & Environmental Medicine, No.1 DaLi Road, Tianjin, 300050, China

    Xinxing Wang, Jingbo Gong, Xiaohua Liu, Rui Zhan, Ruirui Kong, Yun Zhao, Di Wan, Xue Leng, Ming Chen & Lingjia Qian

Authors
  1. Xinxing Wang
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  2. Jingbo Gong
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  3. Xiaohua Liu
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  4. Rui Zhan
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  5. Ruirui Kong
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  6. Yun Zhao
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  7. Di Wan
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  8. Xue Leng
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  9. Ming Chen
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  10. Lingjia Qian
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Corresponding author

Correspondence to Lingjia Qian.

Additional information

Xinxing Wang and Jingbo Gong contributed equally to this work.

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Wang, X., Gong, J., Liu, X. et al. Expression of uncoupling protein 3 in mitochondria protects against stress-induced myocardial injury: a proteomic study. Cell Stress and Chaperones 15, 771–779 (2010). https://doi.org/10.1007/s12192-010-0185-y

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  • DOI: https://doi.org/10.1007/s12192-010-0185-y

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