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Protective effect of carbon monoxide pre-conditioning on LPS-induced endothelial cell stress

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Cell Stress and Chaperones Aims and scope

Abstract

Increasing evidence indicates that carbon monoxide (CO) may protect against several diseases including sepsis. The ability of CO pre-treatment to provide good pre-conditioning against lipopolysaccharide (LPS)-induced injury was tested using an in vitro model of primary culture of porcine aortic endothelial cells (pAEC). pAEC were exposed to CO (250 ppm) or air for 1 h prior to the addition of LPS (10 μg/ml). Hsp70, HO-1, and Egr-1 protein levels were determined as well as vascular endothelial growth factor (VEGF) secretion after 4, 7, and 15 h. The effect of CO on LPS-induced apoptosis was also detected at 15 h. CO pre-treatment before the addition of LPS, significantly reduced LPS-induced apoptosis. LPS induced an increase in the level of VEGF in culture media after 7 and 15 h, and a larger increase was detected in CO pre-treated cells. In addition, CO pre-treatment reduced LPS-induced Hsp70, HO-1, and Egr-1 protein expression. In conclusion, CO treatment seems to provide a good pre-conditioning for the prevention of LPS-induced endothelial injury.

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Acknowledgments

This work was supported by RFO 60% UNIBO and MIUR-PRIN grants.

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Authors and Affiliations

  1. Department of Veterinary Morphophysiology and Animal Production, DIMORFIPA, University of Bologna, Bologna, Italy

    Chiara Bernardini, Augusta Zannoni, Maria Laura Bacci & Monica Forni

  2. Chiara Bernardini Via Tolara di Sopra 50, 40064, Ozzano dell’Emilia, Bologna, Italy

    Chiara Bernardini

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  1. Chiara Bernardini
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  2. Augusta Zannoni
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  3. Maria Laura Bacci
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  4. Monica Forni
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Correspondence to Chiara Bernardini.

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Bernardini, C., Zannoni, A., Bacci, M.L. et al. Protective effect of carbon monoxide pre-conditioning on LPS-induced endothelial cell stress. Cell Stress and Chaperones 15, 219–224 (2010). https://doi.org/10.1007/s12192-009-0136-7

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  • DOI: https://doi.org/10.1007/s12192-009-0136-7

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