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Low concentration of GA activates a preconditioning response in HepG2 cells during oxidative stress—roles of Hsp90 and vimentin

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Cell Stress and Chaperones Aims and scope

Abstract

Oxidative stress can be a significant cause of cell death and apoptosis. We performed studies in HepG2 cells to explore whether prior exposure to oxidative stress (“oxidative preconditioning”) and geldanamycin (GA) treatment can protect the cell from damage caused by subsequent oxidative insults. The cells were treated with 10 nM GA for 24 h before oxidative stress. Oxidative preconditioning was achieved by 2 h exposures to H2O2 (50 μM) separated by a 10-h recovery period in normal culture medium. Oxidative stress was induced by exposure to 500 μM H2O2 for 24 h. The effects of GA and oxidative preconditioning were investigated on the formation of Hsp90, vimentin, insoluble vimentin aggregates, and cleavage of vimentin in a cell culture model of oxidative stress. GA treatment leads to enhanced expression of Hsp90 and vimentin and to inhibition of vimentin protein aggregation. Similar results were obtained by oxidative preconditioning. It is confirmed that low concentrations of GA protected HepG2 cells from subsequent oxidative stress by increasing the levels of Hsp90 and by alleviating the extent of cell apoptosis induced by oxidative stress, which is similar to oxidative preconditioning. However, in contrast to preconditioning, GA treatment obviously changed binding activity of Hsp90 to vimentin cleavages. All the above indicated that low concentrations of GA treatment triggered cell protection from oxidative stress. Both the level of Hsp90 and its ability to bind with vimentin were changed by low concentrations of GA and might contribute to oxidative stress protection.

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Abbreviations

17-AAG:

17-Allylamino-17-demethoxygeldanamycin

GA:

Geldanamycin

Hsp90:

Heat shock protein 90

DMSO:

Dimethyl sulfoxide

FITC:

Fluorescein isothiocyanate

GA:

Geldanamycin

H2O2 :

Hydrogen peroxide

Hsp:

Heat shock protein

MTT:

3[4,5-dimethylthiazole-2-yl]2,5-diphenyltetrazolium bromide

PBS:

Phosphate-buffered saline

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (30500580), the National Natural Science Foundation of Guangdong Province (5300465), and the National Basic Research Program of China, No. 2006CB504100.

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  1. School of Public Health & Tropical Medicine, Southern Medical University, Guangzhou, 510515, China

    Xuemei Chen, Hongyun Kang & Fei Zou

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  1. Xuemei Chen
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  2. Hongyun Kang
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  3. Fei Zou
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Correspondence to Fei Zou.

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Chen, X., Kang, H. & Zou, F. Low concentration of GA activates a preconditioning response in HepG2 cells during oxidative stress—roles of Hsp90 and vimentin. Cell Stress and Chaperones 14, 381–389 (2009). https://doi.org/10.1007/s12192-008-0092-7

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