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Comparison of the effects of 40% oxygen and two atmospheric absolute air pressure conditions on stress-induced premature senescence of normal human diploid fibroblasts

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Cell Stress and Chaperones Aims and scope

Abstract

The pressure during hyperbaric oxygen treatment may increase oxygen toxicity via an augmented oxygen pressure in the gas. Nevertheless, only a few reports have been published on the effect of cells grown under 2 atmospheric absolute (ATA) pressure. To evaluate the effect of pressure on oxygen toxicity and to study effects in addition to oxygen toxicity, we designed an experiment to compare the effects of normobaric mild hyperoxia (NMH, 40% oxygen) and hyperbaric air condition (HA, air with 2 ATA) on human diploid fibroblasts (HDF) in a hyperbaric incubator. HDFs in both the NMH and the HA condition had a similar oxidative stress response and exhibited premature senescence. To investigate differences in gene profiling in cells grown in the NMH and HA conditions, samples from cells exposed to each condition were applied to microarrays. We found no expression difference in genes related to aging and deoxyribonucleic acid damage, but the expression of genes including cell adhesion, stress response, and transcription were significantly increased in fibroblasts that were responsive to pressure. Among 26 statistically reliable genes, the expression of apoptosis related genes such as ADAM22, Bax, BCL2L14, and UBD, as well as tumor suppressor-related genes like Axin2 and ATF, and also mitogen-activated protein kinase-related genes like mitogen-activated protein kinase kinase kinase 1, histamine receptor, and RAB24, were significantly changed in cells responsive to pressure-induced oxidative stress.

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Acknowledgments

This research was supported by the grant from the Medical Research Center for Environmental Toxicogenomic and Proteomics (R13-2003-016-00000-0), funded by Korea Science and Engineering Foundation and Ministry of Science and Technology.

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Authors and Affiliations

  1. Department of Preventive Medicine and Medical Research Center for Environmental Toxico-Genomics and Proteomics, College of Medicine, Korea University, Anam-dong 5ga 126-1, Seongbuk-gu, Seoul, 136-705, South Korea

    Sangnam Oh, Eunil Lee, Joohyun Lee, Yongchul Lim & Joonhee Kim

  2. Cellular and Developmental Biology, Division of Brain Korea 21 Program for Biomedical Science, Korea University, Anam-dong 5ga 126-1, Seongbuk-gu, Seoul, 136-705, South Korea

    Sangnam Oh, Eunil Lee & Yongchul Lim

  3. Postgraduate Studies of Public Health, Graduate School, Korea University, Anam-dong 5ga 126-1, Seongbuk-gu, Seoul, 136-705, South Korea

    Eunil Lee & Joohyun Lee

  4. Korea Research Institute of Standards and Science, Yuseng, Deajeon, 305-340, Korea

    Samyong Woo

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  1. Sangnam Oh
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  2. Eunil Lee
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Correspondence to Eunil Lee.

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Oh, S., Lee, E., Lee, J. et al. Comparison of the effects of 40% oxygen and two atmospheric absolute air pressure conditions on stress-induced premature senescence of normal human diploid fibroblasts. Cell Stress and Chaperones 13, 447–458 (2008). https://doi.org/10.1007/s12192-008-0041-5

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  • DOI: https://doi.org/10.1007/s12192-008-0041-5

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