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New candidate genes for heat resistance in Drosophila melanogaster are regulated by HSF

Cell Stress and Chaperones volume 13, pages 177–182 (2008)Cite this article

Abstract

The cellular heat stress response is well studied in Drosophila in respect to the role of heat shock proteins (Hsp). Hsps are molecular chaperones, highly expressed during and after exposure to numerous stress types. Hsps are all regulated by a common transcription factor, the heat shock factor (HSF), and it is known that HSF is controlling other, so far uncharacterised, heat-responsive genes. In this study, we investigate whether novel candidate genes for heat resistance, identified by microarray experiments, are regulated by HSF. The microarray experiments recently identified several strongly upregulated genes in response to a short, non-lethal heat treatment in Drosophila melanogaster. To test whether or not a subset of these genes are HSF-induced, we studied 11 currently unannotated genes using quantitative polymerase chain reaction on HSF mutant flies with a non-functional HSF at elevated temperatures. We found indication of HSF regulation in most of the studied genes, suggesting a role of these unknown genes in heat tolerance. Surprisingly, some of the genes seemed to be upregulated independent of HSF function. The high induction in response to heat, which mimics the expression profile of Hsps, implies a role in the cellular heat response of these genes as well.

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Acknowledgements

We thank Jesper Sørensen for assistance with the fly strains and Kamilla Pedersen, Torsten Kristensen, Cino Pertoldi and Pernille Sarup for helpful suggestions to the manuscript. The study was supported by a center grant from the Natural Sciences Research Council and a grant from the Novo Nordisk Foundation to VL. LTJ was supported by a stipend from the Faculty of Science, University of Aarhus.

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Affiliations

  1. Department of Biological Sciences, University of Aarhus, Ny Munkegade, Bldg. 1540, 8000, Aarhus C, Denmark

    Louise Toft Jensen & Volker Loeschcke

  2. Department of Molecular Biology, University of Aarhus, C. F. Møllers Allé, Bldg. 1130, 8000, Aarhus C, Denmark

    Morten Muhlig Nielsen

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  1. Louise Toft Jensen
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  2. Morten Muhlig Nielsen
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  3. Volker Loeschcke
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Correspondence to Louise Toft Jensen.

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Jensen, L.T., Nielsen, M.M. & Loeschcke, V. New candidate genes for heat resistance in Drosophila melanogaster are regulated by HSF. Cell Stress and Chaperones 13, 177–182 (2008). https://doi.org/10.1007/s12192-008-0020-x

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  • DOI: https://doi.org/10.1007/s12192-008-0020-x

Keywords

  • Heat Resistance
  • Heat Shock Factor
  • Heat Induction
  • Follow Heat Shock
  • Unannotated Gene