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Comparative evaluation of reagents for measuring protein S activity: possibility of harmonization

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Abstract

Patients with congenital protein S (PS) deficiency show a hereditary predisposition for thrombosis, and PS deficiency is prevalent among Japanese populations. Diagnosis is based on symptoms of thrombosis and reduced PS activity. Three reagents that use different measurement principles for determining PS activity are available in Japan. This study aimed to confirm the possibility of harmonization of these three reagents to establish a universal standard for PS activity in Japanese populations. Commercial normal plasma and plasma samples obtained from healthy individuals and healthy pregnant women were tested at three facilities using three reagents for measuring PS: STA-Staclot Protein S (STA-PS), HemosIL Protein S (Clotting) (IL-PS), and a total PS assay (SNT-PS). The within-run precision of each reagent was good, as each had a coefficient of variation of ≤ 3.8%. The dilution linearity for each reagent was also good. The correlation coefficient was 0.94 for STA-PS vs. IL-PS, 0.93 for SNT-PS vs. STA-PS, and 0.90 for SNT-PS vs. IL-PS, indicating a good correlation. Although the three reagents available in Japan for measuring PS activity use different measurement methods, each showed good performance, and large differences were not observed between the obtained values. Harmonization among them appears possible.

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Acknowledgements

We thank Hiroko Juraku and Kyoko Kumagai for helping with the PS activity measurements. We also thank Yayoi Ishida for the administrative support in writing the manuscript. The manuscript was proofread for language usage by Enago (www.enago.jp) before submission.

Funding

The authors did not receive support from any organization for the submitted work.

Author information

Authors and Affiliations

  1. Department of Internal Medicine, School of Dentistry, Health Sciences University of Hokkaido, 1757-Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan

    Masahiro Ieko, Kazumasa Ohmura & Nobuhiko Takahashi

  2. Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital, Fukuoka, 812-0054, Japan

    Taeko Hotta, Kumiko Watanabe & Dongchon Kang

  3. Department of Obstetrics and Gynecology, Aiiku Maternal and Child Health Center, Tokyo, 105-8321, Japan

    Tomoko Adachi & Sawako Takeuchi

  4. Department of Clinical Laboratory, Health Sciences University of Hokkaido Hospital, Sapporo, 002-8072, Japan

    Sumiyoshi Naito & Mika Yoshida

  5. Department of Clinical Laboratory Science, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, 920-0942, Japan

    Eriko Morishita

  6. Graduate School of Health and Nutrition Sciences, Nakamura Gakuen University, Fukuoka, 814-0198, Japan

    Hiroko Tsuda

  7. Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 819-0395, Japan

    Dongchon Kang

Authors
  1. Masahiro Ieko
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  2. Taeko Hotta
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  3. Kumiko Watanabe
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  4. Tomoko Adachi
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  5. Sawako Takeuchi
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  6. Sumiyoshi Naito
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  7. Mika Yoshida
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  8. Kazumasa Ohmura
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  9. Nobuhiko Takahashi
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  10. Eriko Morishita
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  11. Hiroko Tsuda
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  12. Dongchon Kang
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Contributions

MI conceived the study, provided research guidance, and wrote the manuscript. EM, HT, and DK interpreted the data and critically revised the manuscript. Data analysis was performed by MI, TH, and TA. Testing was done by SN, MY, TH, KW, and ST. Patient enrolment was done by KO, NT, and TA. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Corresponding author

Correspondence to Masahiro Ieko.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval

This study was approved by the Ethics Committee of the School of Dentistry at the Health Sciences University of Hokkaido (No. 148 and 192) and the Medical Ethics Committee of the Aiiku Maternal and Child Health Center (No. 152).

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All subjects provided informed consent before study participation.

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Ieko, M., Hotta, T., Watanabe, K. et al. Comparative evaluation of reagents for measuring protein S activity: possibility of harmonization. Int J Hematol 113, 530–536 (2021). https://doi.org/10.1007/s12185-020-03049-8

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  • DOI: https://doi.org/10.1007/s12185-020-03049-8

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