miR-150 regulates B lymphocyte in autoimmune hemolytic anemia/Evans syndrome by c-Myb
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The objective of the study was to study the regulation of B lymphocytes in patients with autoimmune hemolytic anemia (AIHA)/Evans syndrome. From October 2015 to May 2016, 35 patients with AIHA/Evans in the Department of Hematology, Tianjin Medical University General Hospital were enrolled into this study. c-Myb mRNA and miR-150 expression in B lymphocytes were measured using real-time PCR (RT-PCR). Correlation between c-Myb and miR-150 and clinical parameters in patients with AIHA/Evans were analyzed. c-Myb mRNA expression in hemolysis patients was significantly higher than in remission patients and CLL patients, negatively correlated with hemoglobin (Hb) level and complement 3(C3) levels, and positively correlated with total bilirubin (TBIL) concentration and indirect bilirubin (IBIL) concentration. miR-150 expression in hemolysis patients was significantly lower than in patients in remission and CLL patients. miR-150 was negatively correlated with TBIL and IBIL, and positively correlated with Hb, C3. c-Myb mRNA expression levels in hemolytic episode patients were negatively correlated with the expression levels of miR-150. The expression of c-Myb, a regulatory factor of B lymphocytes, is increased in B lymphocytes of AIHA/Evans patients, while miR-150 expression is decreased. c-Myb was negatively correlated with miR-150.
KeywordsAutoimmune hemolytic anemia B lymphocyte c-Myb miR-150
This work was supported by grants from Application Foundation and Advanced Technology Research Project of Tianjin (15JCYBJC27300), National Natural Science Foundation of China (81370607), Science and Technology Foundation of Tianjin Health Bureau (09KZ108).
XLM and SX participated in manuscript writing. SZH and XLM designed this study. LH and WHQ collected the cases. XWY, ZHL, QYY and ZMJ took part in the study.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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