International Journal of Hematology

, Volume 107, Issue 6, pp 666–672 | Cite as

miR-150 regulates B lymphocyte in autoimmune hemolytic anemia/Evans syndrome by c-Myb

  • Limin XingEmail author
  • Wenyan Xu
  • Yingying Qu
  • Manjun Zhao
  • Hongli Zhu
  • Hong Liu
  • Huaquan Wang
  • Xin Su
  • Zonghong ShaoEmail author
Original Article


The objective of the study was to study the regulation of B lymphocytes in patients with autoimmune hemolytic anemia (AIHA)/Evans syndrome. From October 2015 to May 2016, 35 patients with AIHA/Evans in the Department of Hematology, Tianjin Medical University General Hospital were enrolled into this study. c-Myb mRNA and miR-150 expression in B lymphocytes were measured using real-time PCR (RT-PCR). Correlation between c-Myb and miR-150 and clinical parameters in patients with AIHA/Evans were analyzed. c-Myb mRNA expression in hemolysis patients was significantly higher than in remission patients and CLL patients, negatively correlated with hemoglobin (Hb) level and complement 3(C3) levels, and positively correlated with total bilirubin (TBIL) concentration and indirect bilirubin (IBIL) concentration. miR-150 expression in hemolysis patients was significantly lower than in patients in remission and CLL patients. miR-150 was negatively correlated with TBIL and IBIL, and positively correlated with Hb, C3. c-Myb mRNA expression levels in hemolytic episode patients were negatively correlated with the expression levels of miR-150. The expression of c-Myb, a regulatory factor of B lymphocytes, is increased in B lymphocytes of AIHA/Evans patients, while miR-150 expression is decreased. c-Myb was negatively correlated with miR-150.


Autoimmune hemolytic anemia B lymphocyte c-Myb miR-150 



This work was supported by grants from Application Foundation and Advanced Technology Research Project of Tianjin (15JCYBJC27300), National Natural Science Foundation of China (81370607), Science and Technology Foundation of Tianjin Health Bureau (09KZ108).

Author contributions

XLM and SX participated in manuscript writing. SZH and XLM designed this study. LH and WHQ collected the cases. XWY, ZHL, QYY and ZMJ took part in the study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    He Y, Jiang X, Chen J. The role of miR-150 in normal and malignant hematopoiesis. Oncogene. 2014;33(30):3887–93.CrossRefPubMedGoogle Scholar
  2. 2.
    Garo LP, Murugaiyan G. Contribution of MicroRNAs to autoimmune diseases. Cell Mol Life Sci. 2016;73(10):2041–51.CrossRefPubMedGoogle Scholar
  3. 3.
    Lin YC, Kuo MW, Yu J, et al. c-Myb is an evolutionary conserved miR-150 target and miR-150/c-Myb interaction is important for embryonic development. Mol Biol Evol. 2008;25(10):2189–98.CrossRefPubMedGoogle Scholar
  4. 4.
    Xiao C, Calado DP, Galler G, et al. MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell. 2007;131(1):146–59.CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Zhinan Z. Criteria of diagnosis and curative effect of hematopathy. 3rd ed. China: Science Press; 2007. p. 68–71.Google Scholar
  6. 6.
    Neff AT. Autoimmune hemolytic anemias. In: Greer JP, Foerster J, Lukens JN, Rodgers GM, Paraskevas F, Glader B, editors. Wintrobe’s clinical hematology. Philadelphia: Lippincott Williams & Wilkins; 2004. p. 1157–82.Google Scholar
  7. 7.
    Li K, Tie H, Hu N, et al. Association of two polymorphisms rs2910164 in miRNA-146a and rs3746444 in miRNA-499 with rheumatoid arthritis: a meta-analysis. Hum Immunol. 2014;75(7):602–8.CrossRefPubMedGoogle Scholar
  8. 8.
    Lai NS, Hui-Chun Y, Tung CH, Huang KY, Huang HB, Ming-Chi L. The role of aberrant expression of T cell miRNAs affected by TNF-α in the immunopathogenesis of rheumatoid arthritis. Arthr Res Ther. 2017;19(1):261.CrossRefGoogle Scholar
  9. 9.
    Fu L, Jin YL, et al. Comprehensive review of genetic association studies and meta-analysis on miRNA polymorphisms and rheumatoid arthritis and systemic lupus erythematosus susceptibility. Hum Immunol. 2016;77(1):1–6.CrossRefPubMedGoogle Scholar
  10. 10.
    Nogales-Gadea G, Ramos-Fransi A, Suárez-Calvet X, et al. Analysis of serum miRNA profiles of myasthenia gravis patients. PLoS One. 2014;9(3):e91927.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Burenbatu, Borjigin M, Eerdunduleng, et al. Profiling of miRNA expression in immune thrombocytopenia patients before and after Qishunbaolier (QSBLE) treatment. Biomed Pharmacother. 2015;75:196–204.CrossRefPubMedGoogle Scholar
  12. 12.
    Ferrer G, Navarro A, Hodgson K, et al. MicroRNA expression in chronic lymphocytic leukemia developing autoimmune hemolytic anemia. Leuk Lymphoma. 2013;54(9):2016–22.CrossRefPubMedGoogle Scholar
  13. 13.
    Stenman G, Andersson MK, Andren Y. New tricks from an old oncogene. Cell Cycle. 2010;9(15):2986–95.CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Stamatopoulos B, Van Damme M, Crompot E, et al. Opposite prognostic significance of cellular and serum circulating MicroRNA-150 in patients with chronic lymphocytic leukemia. Mol Med. 2015;21:123–33.CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Thomas MD, Kremer CS, Ravichandran KS. c-Myb is critical for B cell development and maintenance of follicular B cells. Immunity. 2005;23:275–86.CrossRefPubMedGoogle Scholar
  16. 16.
    Greig KT, de Graaf CA, Murphy JM, et al. Critical roles for c-Myb in lymphoid priming and early B-cell development. Blood. 2010;115:2796–805.CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Wei H, Ding XC, Ren JG, et al. A murine model for human immune thrombocytopenic purpura and comparative analysis of multiple gene expression in bone marrow and spleen. J Genet Genomics. 2008;35(11):665–71.CrossRefPubMedGoogle Scholar

Copyright information

© The Japanese Society of Hematology 2018

Authors and Affiliations

  1. 1.Hematology Department of General HospitalTianjin Medical UniversityTianjinChina
  2. 2.Hematology department of the 1st People’s Hospital of JiningShandongChina
  3. 3.Shanghai Tenth People’s HospitalTongji University School of MedicineShanghaiChina

Personalised recommendations