Abstract
Although several DNA-based human platelet antigens (HPA) typing techniques, such as PCR-SSP and PCR-SSO, have been established, the typing errors and the lack of interlaboratory reproducibility are still the issues of concerns. In the present study, polymerase chain reaction primers were designed for identification of all the phenotypically different HPA-1 to HPA-17w types by sequencing-based typing (SBT) method using genomic DNA samples. No discrepancies were observed between PCR-SSP typing and SBT typing in typing a panel of HPA-typed platelet donors that included all common HPA types and the rare HPA-1b, 2b, 3b, and 6bw homozygous donors.
References
Metcalfe P, Watkins NA, Ouwehand WH, et al. Nomenclature of human platelet antigens. Vox Sang. 2003;85:240–5. doi:10.1046/j.1423-0410.2003.00331.x.
Hurd CM, Cavanagh G, Schuh A, Ouwehand WH, Metcalfe P. Genotyping for platelet-specific antigens: techniques for the detection of single nucleotide polymorphisms. Vox Sang. 2002;83:1–12. doi:10.1046/j.1423-0410.2002.00187.x.
Ficko T, Galvani V, Rupreht R, Dovc T, Rozman P. Real-time PCR genotyping of human platelet alloantigens HPA-1, HPA-2, HPA-3 and HPA-5 is superior to the standard PCR-SSP method. Transfus Med. 2004;14:425–32. doi:10.1111/j.1365-3148.2004.00538.x.
Beiboer SH, Wieringa-Jelsma T, Maaskant-Van Wijk PA, et al. Rapid genotyping of blood group antigens by multiplex polymerase chain reaction and DNA microarray hybridization. Transfusion. 2005;45:667–79. doi:10.1111/j.1537-2995.2005.04319.x.
Stafford P, Garner SF, Rankin A, Kekomaki R, Watkins NA, Ouwehand WH. A single-nucleotide polymorphism in the human ITGB3 gene is associated with the platelet-specific alloantigen Va (HPA-17bw) involved in fetal maternal alloimmune thrombocytopenia. Transfusion. 2008;48:1432–8. doi:10.1111/j.1537-2995.2008.01737.x.
Bessos H, Wilson DW, Metcalfe P, Allen D, Urbaniak SJ. Report on the 12th International Society of Blood Transfusion platelet immunology workshop. Vox Sang. 2005;89:105–13. doi:10.1111/j.1423-0410.2005.00672.x.
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Wu, Gg., Tang, Qm., Shen, Wd. et al. DNA sequencing-based typing of HPA-1 to HPA-17w systems. Int J Hematol 88, 268–271 (2008). https://doi.org/10.1007/s12185-008-0164-6
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DOI: https://doi.org/10.1007/s12185-008-0164-6