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Matrix metalloproteinase 9 expression: new regulatory elements

  • Published:
Journal of Ocular Biology, Diseases, and Informatics

Abstract

Retinal ganglion cells apoptosis is linked to matrix metalloproteinase 9 (MMP-9) controlled changes of extracellular matrix. Abnormal expression of MMP-9 is associated with glaucomatous alterations. Thus, the knowledge of MMP-9 regulation is important for the understanding the pathogenesis of glaucoma. Here, we investigated the role of 3′-untranslated regions (3′-UTR) and microRNAs in MMP-9 regulation. We used in vitro mutagenesis and Luc reporter system to identify regulatory elements in the 3′-UTR of MMP-9. microRNAs were analyzed by qRT-PCR, and their role was investigated with inhibitors and mimics. We identified targets for miRNAs in 3′-UTR of MMP-9 involved in the regulation of MMP-9 expression. We then isolated miRNAs from the optic nerve A7 astrocytes and 293 T cells and confirmed the role of mi340 in the regulation using specific inhibitors and mimics. The results obtained show a new miRNA-mediated mechanism of MMP-9 expression regulation.

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Acknowledgements

This study was supported by VA Merit review grant and The Glaucoma Foundation grant.

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Authors and Affiliations

  1. Laboratory of Retinal Biology, VA Medical Center, 4801 E Linwood Blvd, Kansas City, MO, 64128, USA

    Irina Surgucheva & Andrei Surguchov

  2. Department of Neurology, Kansas University Medical Center, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA

    Irina Surgucheva & Andrei Surguchov

  3. Ocean Ridge Biosciences, 10475 Riverside Drive, Palm Beach Gardens, FL, 33410-4208, USA

    Kumaravel Chidambaram & David A. Willoughby

Authors
  1. Irina Surgucheva
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  2. Kumaravel Chidambaram
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  3. David A. Willoughby
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  4. Andrei Surguchov
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Correspondence to Andrei Surguchov.

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Surgucheva, I., Chidambaram, K., Willoughby, D.A. et al. Matrix metalloproteinase 9 expression: new regulatory elements. j ocul biol dis inform 3, 41–52 (2010). https://doi.org/10.1007/s12177-010-9054-2

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  • DOI: https://doi.org/10.1007/s12177-010-9054-2

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