Abstract
Rice grains represent a significant source of carbohydrates and contain trace amounts of other essential nutritional components, including proteins and lipids. The aromatic attributes of rice germplasm typically linked to the existence of aromatic amino acids which are often identified through conventional sensory time intensive assessment methods with statistical significance of p-value of 0.518 × 10−7 and SD of 1.74. Similarly, Raman spectroscopy is explored for the rapid analysis of intact brown rice kernels. In this study, we have examined the efficacy of Raman spectroscopy in conjunction with chemometric approaches for the identification of rice germplasm. Additionally, the assessment of aromatic attributes yielded an accuracy rate of 78%. Notably, the spectral features at ~1603 cm−1 and ~1633 cm−1 within the fingerprint region played a role in detecting the presence of fibre and amide content. Moreover, low standard error of spectra confirms accuracy as statistical significance. The Raman spectroscopy facilitated the revelation of the nutritional profile of rice; certain Raman bands could have been obstructed due to the opaque thick outer layer characteristic of brown rice kernels.
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Acknowledgements
SK is grateful to the Chhatrapati Shahu Maharaj National Research Fellowship-2020 (CSMNRF-2020) provided by SARTHI, Pune (An Autonomous Institute of Government of Maharashtra), for the doctoral research. SK is grateful to Bhartiya Agro-Industries Foundation (BAIF), Pune, for providing the material.
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SB conceptualized the work; SB and CMK designed the research; SK and AH conducted Raman experiments; SK analysed the data; SK and PJ wrote the paper; SB, RS, AH, SS, and CMK read the content of the article.
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Saurabh Kadam declares that he has no conflict of interest. Priyanka Jadhav declares that she has no conflict of interest. Rajshri Singh declares that she has no conflict of interest. Arti Hole declares that she has no conflict of interest. Santosh Sawardekar declares that he has no conflict of interest. C. Murali Krishna declares that he has no conflict of interest. Sagar Barage declares that he has no conflict of interest.
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Kadam, S., Jadhav, P., Singh, R. et al. Raman Spectroscopic Characterization of Local Rice Germplasm from Konkan Region of Maharashtra. Food Anal. Methods 17, 426–435 (2024). https://doi.org/10.1007/s12161-024-02573-1
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DOI: https://doi.org/10.1007/s12161-024-02573-1