Abstract
The optimum saponification extracting conditions of sterol of Pleurotus tuber-regium (Fr.) Sing Sclerotium (PTRSS) with ultrasonic were studied by the Response Surface Methodology (RSM). The results of optimum extraction conditions from Box-Behnken model were as follow: ultrasonic time, 48 min; solid-to-liquid ratio, 1:12.5 g/mL; saponification temperature, 81 ℃; saponification concentration, 0.61 mol/L. Under these conditions, the sterol content was 497.89 ± 0.75 µg/g. Fourier transform infrared spectroscopy was used to analyze the structures of the substance and shows that it has the characteristic peak of sterol. In a certain concentration range, the dose-effect relationship between sterol of PTRSS and antioxidant activity. The binding capacity of 4 mg/mL sterol PTRSS to sodium cholate, sodium glycylcholate and sodium taurocholate in vitro was 36.05 ± 0.76%, 29.41 ± 0.64%, 8.90 ± 0.34%, respectively, indicating that it possessed good hypolipodemic effect in vitro.
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This work was supported by the Basic Public Welfare Research Program of Zhejiang Province (No. LGN22C200001) and the school-level Student Research Training Program of Zhejiang A & F University (No. S202210341206).
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Chao and Yuan wrote the main manuscript text. Yuanhong and Jianing prepared figures ang tables. All authors reviewed the manuscript.
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Liu, Y., Wang, C., Lan, Y. et al. Optimization of Extraction Process of Sterol of Pleurotus Tuber-Regium (Fr.) Sing Sclerotium and Their Antioxidant and Hypolipidemic Effects. Food Anal. Methods 17, 349–357 (2024). https://doi.org/10.1007/s12161-024-02572-2
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DOI: https://doi.org/10.1007/s12161-024-02572-2