Abstract
Paraquat dichloride is a widely used herbicide that poses a significant threat to human health. Its prohibition in several countries highlights the need for the development of a highly sensitive device capable of detecting minimum concentrations of this herbicide in food matrices. In this study, we developed a lateral flow test strip using gold nanoparticles conjugated to anti-paraquat antibodies for the visual and quantitative detection of paraquat in food samples. Through the optimization of various critical factors that affect the sensitivity of the test, we achieved visual and quantitative detection limits of 4 ppb and 1.69 ppb, respectively. These values fall below the maximum residue limits allowed for paraquat dichloride in the USA and the European Union. Results can be obtained in as little as 20 min and the test exhibited good repeatability, with a coefficient of variation of 3.27%. There was no cross reaction with the commonly used pesticides chlorpyrifos and glyphosate, indicating good selectivity. The viability of the test was further confirmed through the analysis of real samples such as soybean, potato, and banana, demonstrating its potential as a rapid and sensitive tool for detecting paraquat residues in food samples.
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References
Bever CS, Adams CA, Hnasko RM, Cheng LW, Stanker LH (2020) Lateral flow immunoassay (LFIA) for the detection of lethal amatoxins from mushrooms. PloS One 15(4):e0231781. https://doi.org/10.1371/journal.pone.0231781
Boušová K, Bruggink C, Godula M, Center S (2017) Fast routine analysis of polar pesticides in foods by suppressed ion chromatography and mass spectrometry. Braz J Anal Chem 4(17):66–78
de Almeida RM, Yonamine M (2007) Gas chromatographic–mass spectrometric method for the determination of the herbicides paraquat and diquat in plasma and urine samples. J Chromatogr B 853(1-2):260–264. https://doi.org/10.1016/j.jchromb.2007.03.026
European Commission (EC). 2022 Food safety. Maximum residue levels. https://ec.europa.eu/food/plants/pesticides/maximum-residue-levels_es. Accessed 10 August 2022 .
Feng R, Wang M, Qian J, He Q, Zhang M, Zhang J et al (2022) Monoclonal antibody-based enzyme-linked immunosorbent assay and lateral flow immunoassay for the rapid screening of paraquat in adulterated herbicides. Microchem J 180:107644. https://doi.org/10.1016/j.microc.2022.107644
Ferrigno PK (2016) Non-antibody protein-based biosensors. Essays Biochem 60(1):19–25. https://doi.org/10.1042/EBC20150003
Fu G, Duan Y, Yi W, Zhang S, Liang W, Li H et al (2022) A simple and reliable paraquat test strip ready for clinical application and field detection. SSRN Electron J:4071338. https://doi.org/10.2139/ssrn.4071338
Huang Y, Zhan H, Bhatt P, Chen S (2019) Paraquat degradation from contaminated environments: current achievements and perspectives. Front Microbiol 10:1754. https://doi.org/10.3389/fmicb.2019.01754
Jiang X, Lillehoj PB (2021) Lateral flow immunochromatographic assay on a single piece of paper. Analyst 146(3):1084–1090. https://doi.org/10.1039/D0AN02073G
Kim JW, Kim DS (2020) Paraquat: toxicology and impacts of its ban on human health and agriculture. Weed Sci 68(3):208–213. https://doi.org/10.1017/wsc.2019.70
Laghrib F, Bakasse M, Lahrich S, El Mhammedi MA (2020) Electrochemical sensors for improved detection of paraquat in food samples: a review. Mater Sci Eng C Mater Biol Appl 107:110349. https://doi.org/10.1016/j.msec.2019.110349
Lan J, Sun W, Chen L, Zhou H, Fan Y, Diao X et al (2020) Simultaneous and rapid detection of carbofuran and 3-hydroxy-carbofuran in water samples and pesticide preparations using lateral-flow immunochromatographic assay. Food Agricult Immunol 31(1):165–175. https://doi.org/10.1080/09540105.2019.1708272
Li Y, Liu L, Kuang H, Xu C (2020) Preparing monoclonal antibodies and developing immunochromatographic strips for paraquat determination in water. Food Chem 311:125897. https://doi.org/10.1016/j.foodchem.2019.125897
Limsuwanchote S, Putalun W, Tanaka H, Morimoto S, Keawpradub N, Wungsintaweekul J (2018) Development of an immunochromatographic strip incorporating anti-mitragynine monoclonal antibody conjugated to colloidal gold for kratom alkaloids detection. Drug Test Anal 10(7):1168–1175. https://doi.org/10.1016/j.jpba.2020.113450
Liu Y, Liu B, Xia L, Yu H, Wang Q, Wu Y (2022) Cationic polyelectrolyte as powerful capture molecule in aptamer-based chromatographic strip for rapid visual detection of paraquat residue in agricultural products. Sens Actuators B 368:132237. https://doi.org/10.1016/j.snb.2022.132237
Oliveira JP, Prado AR, Keijok WJ, Ribeiro MR, Pontes MJ, Nogueira BV, Guimarães MCC (2020) A helpful method for controlled synthesis of monodisperse gold nanoparticles through response surface modeling. Arabian J Chem 13(1):216–226. https://doi.org/10.1016/j.arabjc.2017.04.003
Oliver C (2010) Conjugation of colloidal gold to proteins. In: Immunocytochemical methods and protocols. Humana Press, pp 369–373. https://doi.org/10.1007/978-1-59745-324-0_39
Oshokoya OO, JiJi RD (2015) Parallel factor analysis of multi-excitation ultraviolet resonance Raman spectra for protein secondary structure determination. Anal Chim Acta 892:59–68. https://doi.org/10.1016/j.aca.2015.08.035
Pavan M (2013) Acute kidney injury following Paraquat poisoning in India. Iran J Kidney Dis 7(1):64
Peiró AM, Zapater P, Alenda C, Ramírez A, Gutiérrez A, Pérez-Mateo M, Such J (2007) Hepatotoxicity related to paraquat and diquat absorption through intact skin. Dig Dis Sci 52(11):3282–3284. https://doi.org/10.1007/s10620-005-9056-2
Pereira RHA, Keijok WJ, Prado AR, de Oliveira JP, Guimarães MCC (2021) Rapid and sensitive detection of ochratoxin A using antibody-conjugated gold nanoparticles based on Localized Surface Plasmon Resonance. Toxicon 199:139–144. https://doi.org/10.1016/j.toxicon.2021.06.012
Rai MK, Das JV, Gupta VK (1997) A sensitive determination of paraquat by spectrophotometry. Talanta 45(2):343–348. https://doi.org/10.1016/S0039-9140(97)00136-7
Raysyan A, Galvidis IA, Schneider RJ, Eremin SA, Burkin MA (2020) Development of a latex particles-based lateral flow immunoassay for group determination of macrolide antibiotics in breast milk. J Pharm Biomed Anal 189:113450. https://doi.org/10.1016/j.jpba.2020.113450
Resolution - RDC No. 177 of 2017. Collegiate Board of the National Health Surveillance Agency. Provides for the ban on the active ingredient paraquat in pesticide products in Brazil. September 21, 2017.
Sajid M, Kawde AN, Daud M (2015) Designs, formats and applications of lateral flow assay: a literature review. J Saudi Chem Soc 19(6):689–705. https://doi.org/10.1016/j.jscs.2014.09.001
Sankari G, Krishnamoorthy E, Jayakumaran S, Gunasekaran S, Priya VV, Subramaniam S et al (2010) Analysis of serum immunoglobulins using Fourier transform infrared spectral measurements. Biol Med 2(3):42–48
Srisrattakarn A, Tippayawat P, Chanawong A, Tavichakorntrakool R, Daduang J, Wonglakorn L, Lulitanond A (2020) Developtment of a prototype lateral flow immunoassay for rapid detection of Staphylococcal protein A in positive blood culture samples. Diagnostics 10(10):794. https://doi.org/10.3390/diagnostics10100794
Subbiah R, Tiwari RR (2021) The herbicide paraquat-induced molecular mechanisms in the development of acute lung injury and lung fibrosis. Crit Rev Toxicol 51(1):36–64. https://doi.org/10.1080/10408444.2020.1864721
Tanner CM, Kamel F, Ross GW, Hoppin JA, Goldman SM, Korell M et al (2011) Rotenone, paraquat, and Parkinson’s disease. Environ Health Perspect 119(6):866–872. https://doi.org/10.1289/ehp.1002839
Tsai WT (2013) A review on environmental exposure and health risks of herbicide paraquat. Toxicol & Environ Chem 95(2):197–206. https://doi.org/10.1080/02772248.2012.761999
Turkevich J, Stevenson PC, Hillier J (1951) A study of the nucleation and growth processes in the synthesis of colloidal gold. Discuss Faraday Soc 11:55–75
United States department of agriculture (USDA). (2022) Maximum residue limits (MRL) database. https://www.fas.usda.gov/maximum-residue-limits-mrl-database. Accessed 10 August 2022.
Wesseling C, De Joode BVW, Ruepert C, León C, Monge P, Hermosillo H, Partanen LJ (2001) Paraquat in developing countries. Int J Occup Environ Health 7(4):275–286. https://doi.org/10.1179/107735201800339209
Wigfield YY, McCormack KA, Grant R (1993) Simultaneous determination of residues of paraquat and diquat in potatoes using high-performance capillary electrophoresis with a ultraviolet detection. J Agric Food Chem 41(12):2315–2318. https://doi.org/10.1021/jf00036a018
Wu B, Song B, Yang H, Huang B, Chi B, Guo Y, Liu H (2013) Central nervous system damage due to acute paraquat poisoning: an experimental study with rat model. Neurotoxicology 35:62–70. https://doi.org/10.1016/j.neuro.2012.12.001
Acknowledgements
This study used the equipment facilities of the Nanomedicine and Nanotoxicology Group and the Laboratory of Cellular Ultrastructure Carlos Alberto Redins. Our thanks to both Labs for providing the equipment and technical support for experiments.
Funding
This study had financial support from the Foundation for the Support of Research and Innovation of Espírito Santo (Grant No 10/2019).
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Conceptualization: JPO; formal analysis and investigation: MDLJ and LACA; writing—original draft preparation: MDLJ, LACA, JPO; writing—review and editing: JPO, FVC, PWPA, and MCCG; and supervision: JPO.
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Jara, M.D.L., Alvarez, L.A.C., Campos, F.V. et al. Development of a Competitive Lateral Flow Assay for Rapid Paraquat Detection. Food Anal. Methods 17, 284–295 (2024). https://doi.org/10.1007/s12161-023-02566-6
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DOI: https://doi.org/10.1007/s12161-023-02566-6