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Development of an Immunochromatographic Strip for the Rapid and Ultrasensitive Detection of Gamithromycin

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Abstract

In this study, we developed a monoclonal antibody (mAb)–based immunochromatographic strip for the detection of gamithromycin (GAM) residues in beef and milk samples. The mAb had a high sensitivity with a 50% inhibitory concentration (IC50) of 0.537 ng/mL, and the cross-reactivity results indicated that the anti-GAM mAb also displayed a high specificity. The immunochromatographic strip gave cutoff values of 2 μg/kg and 5 ng/mL for beef and milk samples, respectively, and the results could be observed within 5 min using the naked eye. Analysis of GAM in beef and milk samples indicated that detection using the immunochromatographic strip was comparable to results using ic-ELISA and LC–MS/MS. Therefore, this immunochromatographic strip represented an accurate, reliable, and rapid method for the detection of GAM in beef and milk samples.

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Funding

This work was partially supported by the National Key Research and Development Program of China (2019YFC1604704).

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Authors and Affiliations

  1. State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People’s Republic of China

    Qianqian Lu, Xinxin Xu, Shanshan Song, Aihong Wu, Liqiang Liu, Hua Kuang & Chuanlai Xu

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  1. Qianqian Lu
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  2. Xinxin Xu
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  3. Shanshan Song
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  4. Aihong Wu
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  5. Liqiang Liu
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  6. Hua Kuang
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  7. Chuanlai Xu
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Corresponding authors

Correspondence to Liqiang Liu or Chuanlai Xu.

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This article does not contain any studies involving human subjects. All animal studies were carried out under the guidance of animal welfare committee of Jiangnan University.

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Conflict of Interest

Qianqian Lu declares no conflict of interest. Xinxin Xu declares no conflict of interest. Shanshan Song declares no conflict of interest. Aihong Wu declares no conflict of interest. Liqiang Liu declares no conflict of interest. Hua Kuang declares no conflict of interest. Chuanlai Xu declares no conflict of interest.

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Lu, Q., Xu, X., Song, S. et al. Development of an Immunochromatographic Strip for the Rapid and Ultrasensitive Detection of Gamithromycin. Food Anal. Methods 15, 598–606 (2022). https://doi.org/10.1007/s12161-021-02146-6

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  • DOI: https://doi.org/10.1007/s12161-021-02146-6

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