Abstract
Since inulin utilization as a sugar or fat replacer, texture modifier, and emulsifier is increasing in the food industry, there is a need for its determination for food control and labeling. This study reports the development and validation of an advanced high-performance liquid chromatographic method with evaporative light-scattering detection (HPLC-ELSD) method for inulin quantification, with optimized extraction conditions (1:10 w/v solid-to-solvent ratio, 1 h/80 °C in a water bath continued with 30 min ultrasonication for the extraction step, and precipitation during the night with four volumes of acetone). The rapid HPLC-ELSD analysis (16 min) has been performed by isocratic elution with water as a mobile phase and optimized detector parameters (temperature of evaporator 80 °C, temperature of nebuliser 80 °C, gas flow rate 1.3 standard liters per minute, detector gain 1). The method was validated in terms of limits of detection and quantification, intra- and inter-day precision and accuracy, and recovery. The proposed method was carried out using a cation exchange (Ag +) column for the first time for the inulin determination, avoiding the hydrolysis step in order to simplify and accelerate the quantification.
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Acknowledgements
The authors refer special thanks to Svetlana Ćujić from PatentCo. for the useful advices, as well as Cluster d.o.o., Serbia, for the technical support.
Funding
This study was funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia (451–03-9/2021–14/200222).
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Investigation (Jelena Perović), methodology (Jelena Perović, Jovana Kojić, Jelena Krulj), validation (Jelena Perović, Jovana Kojić, Jelena Krulj), writing the original draft (Jelena Perović), supervision (Jovana Kojić, Jelena Krulj, Vesna Tumbas Šaponjac, Nebojša Ilić, Marija Bodroža-Solarov), formal analysis (Lato Pezo), conceptualization (Lato Pezo, Vesna Tumbas Šaponjac, Nebojša Ilić), visualization (Vesna Tumbas Šaponjac, Nebojša Ilić), project administration (Marija Bodroža-Solarov), and resources (Marija Bodroža-Solarov).
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Jelena Perović declares that she has no conflict of interest. Jovana Kojić declares that she has no conflict of interest. Jelena Krulj declares that she has no conflict of interest. Lato Pezo declares that he has no conflict of interest. Vesna Tumbas Šaponjac declares that she has no conflict of interest. Nebojša Ilić declares that he has no conflict of interest. Marija Bodroža-Solarov declares that she has no conflict of interest.
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Perović, J., Kojić, J., Krulj, J. et al. Inulin Determination by an Improved HPLC-ELSD Method. Food Anal. Methods 15, 1001–1010 (2022). https://doi.org/10.1007/s12161-021-02140-y
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DOI: https://doi.org/10.1007/s12161-021-02140-y