Abstract
Three novel derivatization reagents, tris(dimethylamino)methane, bis(dimethylamino)methoxymethane, and tert-butoxybis(dimethylamino)methane, were utilized for the simultaneous determination of nine perfluoroalkyl carboxylic acids (PFCAs) by gas chromatography-mass spectrometry (GC–MS). PFCAs were extracted from Chinese wolfberry and soybean by the extractant of ACN/water, and cleaned up on a WAX cartridge, then derivatized with 20 μL of derivatization reagent at 35 °C for 30 min, and finally analyzed by GC–MS. Several parameters, including the amount of derivatization reagents, derivatization temperature and time, as well as sample extraction and clean-up conditions, were optimized and the method was validated in Chinese wolfberry and soybean matrices. The proposed method showed low method quantification limits (MQLs) ranged from 0.25 to 0.40 ng/g and obtained matrix effect (|ME%|) ≤ 20%, which showed negligible matrix influence. The recoveries calculated at the spike levels ranged from 49.3 to 96.3% with relative standard deviations (RSDs) ranged from 1.2 to 14.7%. The proposed method based on a novel derivatization shows the advantages of fast and sensitive for the determination PFCAs in Chinese wolfberry and soybean.
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This study was supported by a National Key Technologies Research and Development Program of China (2017YFF0211304).
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Yongyan Ji declares that she has no conflict of interest. Yanzhong Cao declares that he has no conflict of interest. Xuezhe Huang declares that she has no conflict of interest. Adan Li declares that she has no conflict of interest. Zongyan Cui declares that he has no conflict of interest.
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Ji, Y., Cao, Y., Huang, X. et al. Simultaneous Determination of Nine Perfluoroalkyl Carboxylic Acids in Chinese Wolfberry and Soybean by Gas Chromatography–Mass Spectrometry with a Novel Derivatization Method . Food Anal. Methods 14, 2430–2437 (2021). https://doi.org/10.1007/s12161-021-02072-7
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DOI: https://doi.org/10.1007/s12161-021-02072-7