Abstract
A new simple and rapid HPLC method was described and validated for simultaneous determination of eight banned dye compounds in corn and olive oil. These dyes are namely Sudan I, II, III, and IV (SI, SII, SIII, SIV); Sudan Orange G (SOG); Sudan Red G ( SRG); Fat Brown B (FBB); and Fat Red 7B (FR7B). The oil samples were dissolved in hexane, followed by liquid-liquid extraction and enrichment with acetonitrile. Then, the samples were cleaned using a PTFE syringe filter and analyzed by high-performance liquid chromatography (HPLC) coupled with a photodiode array detector (DAD) for separation, identification, and quantification of these dyes. The chromatographic separations were carried out using an ODS column under an isocratic mode using a mixture of acetonitrile, isopropanol, and water. The method was linear for all targeted compounds over the range of 0.25–50 mg/kg. All correlation recoveries were in the range 87–106% with a precision (RSD) less than 7%. Results of the twenty samples (ten corn oil and the ten olive oil samples), collected randomly from Jordanian markets during February 2018, were lower than the detection limits for the eight compounds.
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Acknowledgements
Analysis were performed at the Pharmaceutical Research Center (PCR) at Jordan University of Science and Technology (JUST), Irbid, Jordan. The authors would like to thank Ashraf AlMutlaq for performing HPLC-DAD and spectophotometeric analysis.
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This research was partially funded by the Deanship of Scientific research-Jordan University of Science and Technology.
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Mousa S. Al-Tarabeen declares that he has no conflict of interest. Abdul-Wahab O. El-Rjoob declares that he has no conflict of interest. Yahya R. Tahboub declares that he has no conflict of interest. Mohammed Y. Rasheed declares that he has no conflict of interest.
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Al-Tarabeen, M.S., El-Rjoob, AW.O., Tahboub, Y.R. et al. Monitoring of Selected Banned Azo Dyes in Edible Oils by High-Performance Liquid Chromatography-Photodiode Array Detector . Food Anal. Methods 14, 2478–2485 (2021). https://doi.org/10.1007/s12161-021-02050-z
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DOI: https://doi.org/10.1007/s12161-021-02050-z