Abstract
Food allergy is an abnormal immune response of the human immune system to some foods, which can cause extremely serious harm to the human body. Therefore, the development of a sensitive and rapid detection approach for food allergens in various food matrices is essential to assist patients in managing their allergies. In this paper, a kind of circular fluorescence probe-mediated isothermal amplification technology (CFPA) was developed and used to detect the peanut allergen encoding gene (Ara h 6) and soybean allergen encoding gene (Gly m Bd 28 K). This novel assay could be finished within 60 min with high sensitivity (10 copies/μL~1 × 107 copies/μL), specificity (no cross-reaction with other allergens), and reproducibility (CV < 0.05). Meanwhile, this method was utilized to detect peanut and soybean allergen encoding genes from different origins, varieties and commercially available foods, and the results consistent with the information of the allergens labeled on the products. We further combined this method with a microfluidic chip to realize the multiple detections of peanut and soybean allergen encoding genes in a portable microfluidic detector.
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Acknowledgments
We are grateful for the kind help of our colleagues in our university research groups. We acknowledge the National Natural Science Foundation of China (21505024, 21427806, 21175029, and 21335002) and the Introduce talents of Fudan University research funding (JIH1615032).
Funding
This study was funded by the National Natural Science Foundation of China (2155024, 21427806, 21175029, 2133502) and the research fund of Fudan University (jih1615032).
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Conceptualization: Qin Chen and Xueen Fang; methodology: Qin Chen and Xueen Fang; formal analysis and investigation: Yongxin Liu; writing—original draft preparation: Yongxin Liu and Xueen Fang; writing—review and editing: Yongxin Liu, Xueen Fang, Bing Niu, and Xiaodong Sun; supervision: Qin Chen, Xueen Fang, and Xiaodong Sun; funding acquisition: Qin Chen and Xueen Fang. All authors have read and agreed to the published version of the manuscript.
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Yongxin Liu declares no conflict of interest. Xueen Fang declares no conflict of interest. Xiaodong Sun declares no conflict of interest. Bing Niu declares no conflict of interest. Qin Chen declares no conflict of interest.
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Liu, Y., Fang, X., Sun, X. et al. Detection of Allergen Genes in Peanut and Soybean by Circular Fluorescence Probe-Mediated Isothermal Amplification. Food Anal. Methods 14, 453–464 (2021). https://doi.org/10.1007/s12161-020-01883-4
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DOI: https://doi.org/10.1007/s12161-020-01883-4