Abstract
Nowadays, the overuse of antibiotics in cow breeding is a common issue worldwide. Real-time monitoring is critical for antibiotic control of dairy products. Herein, we report a rapid detection method for the duplex detection of two beta-lactam antibiotics (cephalexin and ampicillin) in milk powder using lateral flow (LF) combined with surface-enhanced Raman spectroscopy (SERS). The duplex detection was accomplished using a single test line loaded with two antigens targeting the two corresponding antibody-gold-Raman reporter conjugates. The obtained SERS spectra contributed by the Raman reporter labeled to the two antibodies were analyzed using both peak height method and multiple linear regression (MLR). A good regression fitting was achieved by these two methods (R2 = 0.99 and 0.98, respectively). The limit of detection (LOD) was 10−4 ng/mL for both peak height method and MRL. This study indicated that LF-SERS is a good candidate for real-time multiplex and quantitative analysis for antibiotics in dairy products.
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09 February 2022
A Correction to this paper has been published: https://doi.org/10.1007/s12161-022-02227-0
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This work was supported by the Shenyang Industrial Science and Technology Program (grant number F11-049-2-00).
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Li, X., Wang, X., Wang, L. et al. Duplex Detection of Antibiotics in Milk Powder Using Lateral-Flow Assay Based on Surface-Enhanced Raman Spectroscopy. Food Anal. Methods 14, 165–171 (2021). https://doi.org/10.1007/s12161-020-01870-9
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DOI: https://doi.org/10.1007/s12161-020-01870-9